The Australian Ultraviolet Radiation and Immunity Study
Not Applicable
Completed
- Conditions
- Impact of solar ultraviolet radiation on human primary immune response to immunisation with a T-cell dependent antigen (Keyhole Limpet Hemocyanin or KLH)Inflammatory and Immune System - Normal development and function of the immune system
- Registration Number
- ACTRN12610000234011
- Lead Sponsor
- Dr Robyn Lucas
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- All
- Target Recruitment
- 210
Inclusion Criteria
* Age 18 - 40 years
* Healthy
* Willing and able to sign informed consent
Exclusion Criteria
* Allergy to shellfish
* Pregnancy or breastfeeding
* Previous KLH vaccination
* Immunosuppressive medications
* Immunosuppressive medical condition
* Recent infection (within 2 weeks of vaccination)
* Recent immunisation with any other vaccine in past 4 weeks
* Photosensitive skin or taking photosensitsing medication
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Humoral response to primary subcutaneous vaccination with Keyhole Limpet Hemocyanin (KLH). KLH-specific immunoglobulins (Ig) (Ig types M, E, G and subsets) will be quantified via enzyme linked immunoassay techniques.[KLH specific antibody titres taken at baseline (immediately prior to vaccination which follows the 5 days of UVR exposure measurement); 1 week and 3 weeks post vaccination]
- Secondary Outcome Measures
Name Time Method Delayed-type hypersensitivity (DTH) response to intra-dermal KLH will be measured in millimetres of skin reaction[Intradermal KLH given at day 21 post vaccination. DTH response read at 48 hours post intradermal KLH.];KLH specific T-cell response will be measured via lymphocyte proliferation assays and cytokine production.[Measured at baseline (immediately prior to vaccination) and day 21.]