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SENTI-202: Off-the-shelf Logic Gated CAR NK Cell Therapy in Adults with CD33 And/or FLT3 Blood Cancers Including AML/MDS

Phase 1
Recruiting
Conditions
AML/MDS
CD33 Expressing Hematological Malignancies
FLT3 Expressing Hematological Malignancies
Interventions
Biological: SENTI-202
Registration Number
NCT06325748
Lead Sponsor
Senti Biosciences
Brief Summary

This is an open-label study of the safety, biodynamics, and anti-cancer activity of SENTI-202 (an off-the-shelf logic gated CAR NK cell therapy) in patients with CD33 and/or FLT3 expressing blood cancers, including AML and MDS.

Detailed Description

This is a dose-finding study of SENTI-202, comprised of an initial dose finding using a modified "3+3" study design to determine the maximum tolerated dose (MTD) and/or recommended phase two dose (RP2D) of SENTI-202 when administered after lymphodepleting chemotherapy (Part 1) followed by disease-specific expansion cohorts at the RP2D (Part 2).

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
21
Inclusion Criteria
  • Subjects with CD33 and/or FLT3 expressing malignancies, including:

    • Relapsed refractory acute myeloid leukemia (AML) with morphologic relapse as defined by ≥5% bone marrow blasts who have received at least 1 prior line, but no more than 3 prior lines of standard anti-AML therapy. Subjects with FLT3-mutated or IDH ½-mutated disease must have received at least one prior targeted therapy.
    • Relapsed refractory myelodysplastic syndrome (MDS) with increased blasts who have received at least 1 prior line, but no more than 2 prior lines of anti-MDS therapy
    • Other hematological malignancies who have received at least 1 prior line of standard of care for the respective disease
    • Documentation of CD33 expression (or FLT3 expression if available) by individual institutional standard of care
  • ECOG performance score of 0-1

  • Adequate organ function including platelet count >20x109/L (platelet transfusion is permitted)

  • Adequate recovery from toxicities from previous cancer treatments, as described in the study protocol

  • Willing and able to provide written informed consent

Exclusion Criteria
  • White blood cell (WBC) count of ≥20×109/L or circulating blasts ≥10×109/L or rapidly progressive/hyperproliferative disease
  • Acute promyelocytic leukemia with t(15;17) (q22;q12) or abnormal promyelocytic leukemia/retinoic acid receptor alpha (APML-RARA)
  • MDS with fibrosis (MDS-f) or known prior history of constitutional conditions/syndromes with chemo-responsive AML
  • Evidence of leukemic meningitis or known active central nervous system disease
  • Presence of extra-medullary disease or myeloid sarcoma alone with no morphologic hematologic relapse
  • Prior use of certain anti-cancer therapies and/or use within a certain number of days prior to SENTI-202 study treatment, as described in the study protocol
  • Hematopoietic cell transplantation (HCT) less than 100 days prior to the first dose of SENTI-202
  • Prior NK cell or CAR T cell therapy at any time
  • Prior donor lymphocyte infusion (DLI), except if after HCT for MRD+ disease
  • Medical conditions or medications prohibited by the study protocol
  • Pregnant or breastfeeding female

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
SENTI-202 CAR NK cell therapySENTI-202Part 1 Dose Finding: Sequential cohorts will receive doses of SENTI-202 using a modified 3+3 study design to determine the recommended phase 2 dose (RP2D). The starting dose will be 1 billion cells. Other doses may be explored depending on study data. Part 2 Cohort Expansion: After determination of the RP2D, additional subjects will be enrolled in disease-specific expansion cohorts at that dose to further explore safety, biodynamics, and anti-cancer activity of SENTI-202
SENTI-202 CAR NK cell therapySENTI-202Part 1 Dose Finding: Sequential cohorts will receive doses of SENTI-202 using a modified 3+3 study design to determine the recommended phase 2 dose (RP2D). The starting dose will be 1 billion cells. Other doses may be explored depending on study data. Part 2 Cohort Expansion: After determination of the RP2D, additional subjects will be enrolled in disease-specific expansion cohorts at that dose to further explore safety, biodynamics, and anti-cancer activity of SENTI-202
Primary Outcome Measures
NameTimeMethod
Safety and tolerability for dose determination of SENTI-202At the end of each treatment cycle (each cycle is 28 days) and through study completion, up to 2 years

Incidence, type, frequency, and severity of adverse events and dose limiting toxicities will be assessed to determine the maximum tolerated dose and/or recommended phase 2 dose and dosing regimen

Secondary Outcome Measures
NameTimeMethod
Anti-cancer activity of SENTI-202Through study completion, up to 2 years

The response rate to SENTI-202 will be measured using clinical measures of benefit as defined by standard consensus criteria for the respective disease

Pharmacokinetic (PK) and pharmacodynamic (PDn) profile of SENTI-202Through study completion, up to 2 years

Levels of circulating SENTI-202 and peripheral cytokine levels will be measured to assess the PK/PDn profile of SENTI-202

Host immune response to SENTI-202Through study completion, up to 2 years

Anti-SENTI-202 immune response and RCR will be measured in blood samples

Trial Locations

Locations (8)

UCLA Medical Center

🇺🇸

Los Angeles, California, United States

Colorado Blood Cancer Institute

🇺🇸

Denver, Colorado, United States

Mayo Clinic

🇺🇸

Jacksonville, Florida, United States

TriStar Bone Marrow Transplant

🇺🇸

Nashville, Tennessee, United States

MD Anderson Cancer Center

🇺🇸

Houston, Texas, United States

Methodist Healthcare

🇺🇸

San Antonio, Texas, United States

Royal Prince Alfred Hospital

🇦🇺

Camperdown, New South Wales, Australia

Peter MacCallum Cancer Center

🇦🇺

Melbourne, Victoria, Australia

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