Molecular Characterization of Innate and Adaptive Immune Responses in Children with Early Childhood Caries - An in vitro Clinico-Immunological Analysis
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 27
- 试验地点
- 1
- 主要终点
- Comparison of immunological and hematological profiles, including PCR-based expression of innate and adaptive immune markers, among children with early childhood caries, early childhood caries with abscess, and caries-free healthy controls at baseline
研究概览
简要总结
This study aims to characterize innate and adaptive immune responses in children with early childhood caries and early childhood caries with abscess in comparison with caries-free healthy children. Peripheral venous blood samples will be collected to evaluate immunological and hematological profiles along with molecular assessment of immune marker expression using PCR. Immunohistochemical analysis and in-silico molecular docking will be performed to further explore immune-related pathways. Demographic and clinical parameters will also be recorded. The findings of this clinico-immunological study are expected to provide insights into host immune responses associated with early childhood caries
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 3.00 Year(s) 至 6.00 Year(s)（—）
- 性别
- All
入选标准
- •Children diagnosed with Early Childhood caries, or ECC with dental abscesses based on clinical and radiographic examination Caries free, age matched healthy children serving as controls Children whose parents/guardians provide informed written consent Children who are systemically healthy at the time of enrollment.
排除标准
- •Children with systemic diseases,immunological disorders , or hematological abnormalities.
- •Children on antibiotic , anti-inflammatory, or immunosuppressive therapy within the preceding 4 weeks Children with acute systemic infections at the time of sample collection Children with a history of bleeding disorders or on medication affecting coagulation Childrens whose parents/guardians do not consent to participation.
结局指标
主要结局
Comparison of immunological and hematological profiles, including PCR-based expression of innate and adaptive immune markers, among children with early childhood caries, early childhood caries with abscess, and caries-free healthy controls at baseline
时间窗: At baseline
次要结局
- Comparsion of demographic and clinical parameters among the study groups.(Assessment of associated clinical signs and symptoms in children with early childhood caries and early childhood caries with abscess.)
研究者
Niveditha R
Saveetha Dental College and Hospitals