Distinct Sources of Supplementary Protein in the Resistance Exercise Training-induced Adaptations

Not Applicable

Completed

• Conditions: Dietary Supplements; Muscle Strength; Muscle, Skeletal; Diet Habit; Resistance Training
• Interventions: Dietary Supplement: Animal protein; Dietary Supplement: Plant Protein

• Registration Number: NCT05710614
• Lead Sponsor: University of Sao Paulo General Hospital

Brief Summary

This is a randomized controlled trial aimed to investigate the effects of different supplementary protein sources on muscle adaptations to resistance exercise. Young, healthy, recreationally active participants consuming an omnivorous diet with protein intake within recommended dietary allowance (RDA) (i.e.; 0.8-1.0 g.kg-1. d-1) will be recruited to undergo a 12-wk supervised resistance training program in combination with the intake of three 15-g daily doses (45g. d-1 total) of either a mixed plant- (i.e.; soy and pea protein) or animal-based (i.e.; whey protein) protein in drink form as a supplementary source of protein to their main meals of the day (i.e.; breakfast, lunch, and dinner). Before (PRE) and after (POST) the 12-wk intervention, participants will be assessed for body composition by dual-energy X-ray absorptiometry (DXA), muscle cross-sectional area (ultrasound) and fiber cross-sectional area (muscle biopsy), and maximal isotonic strength (1RM). Training consists of a resistance training (RT) program individually supervised by a researcher blinded to treatment in a laboratorial setting. Assessments will also be conducted in a blinded fashion.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2023-01-20
• Study First Submitted QC: 2023-01-30
• Study First Posted: 2023-02-02
• Study Start: 2023-08-01
• Primary Completion: 2024-11-27
• Study Completion: 2024-11-27
• Status Verified: 2024-12
• Last Update Submitted: 2024-12-03
• Last Update Posted: 2024-12-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 40

Inclusion Criteria

• Physically active (according to the International Physical Activity Questionnaire (IPAQ)
• Absence of any chronic condition that could preclude participation in a RT program or physical testing
• Habitual protein consumption within RDA ≥ 0.8 ≤1.0 g.kg-1. d-1

Exclusion Criteria

• Prior history of anabolic steroids use, current or previous (≤ 3 m)
• Use of ergogenic or protein-based supplements, current or previous (< 1 y)
• Engagement in energy-restricted diets

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
animal protein	Animal protein	Whey protein will be used as the active comparator for animal protein arm
plant protein	Plant Protein	A combination of pea and soy protein will be used as the experimental arm

Primary Outcome Measures

Name	Time	Method
vastus lateralis muscle cross sectional area	Ultrasound images will be obtained both at baseline (before the comencement of the study) and immediately after the 12-week intervention period	the investigators used ultrasound measures of the vastus lateralis cross sectional area as the primary outcome

Secondary Outcome Measures

Name	Time	Method
lean mass	DXA scans will be taken both at baseline (before the comencement of the study) and immediately after the 12-week intervention period	total body and lower limb lean mass assessed by DXA
fiber cross sectional area	Fiber cross sectional areas will be measured both at baseline (before the comencement of the study) and immediately after the 12-week intervention period	vastus lateralis fiber cross sectional area from muscle biopsy samples
maximal isotonic strength	1-RM tests will be taken both at baseline (before the comencement of the study) and immediately after the 12-week intervention period	lower-limb maximal isotonic strength will be assessed in the leg-press exercise by means of the one-repetition-maximum test (1-RM test)

Trial Locations

Locations (1)

University of Sao Paulo; 🇧🇷 São Paulo, Brazil