Mixed Plant- vs. Animal-based Supplementary Protein to Support Muscle Adaptations to Resistance Exercise: a Randomized Clinical Trial
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Enrollment
- 40
- Locations
- 2
- Primary Endpoint
- vastus lateralis muscle cross sectional area
Study Overview
Brief Summary
This is a randomized controlled trial aimed to investigate the effects of different supplementary protein sources on muscle adaptations to resistance exercise. Young, healthy, recreationally active participants consuming an omnivorous diet with protein intake within recommended dietary allowance (RDA) (i.e.; 0.8-1.0 g.kg-1. d-1) will be recruited to undergo a 12-wk supervised resistance training program in combination with the intake of three 15-g daily doses (45g. d-1 total) of either a mixed plant- (i.e.; soy and pea protein) or animal-based (i.e.; whey protein) protein in drink form as a supplementary source of protein to their main meals of the day (i.e.; breakfast, lunch, and dinner). Before (PRE) and after (POST) the 12-wk intervention, participants will be assessed for body composition by dual-energy X-ray absorptiometry (DXA), muscle cross-sectional area (ultrasound) and fiber cross-sectional area (muscle biopsy), and maximal isotonic strength (1RM). Training consists of a resistance training (RT) program individually supervised by a researcher blinded to treatment in a laboratorial setting. Assessments will also be conducted in a blinded fashion.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Treatment
- Masking
- Triple (Participant, Investigator, Outcomes Assessor)
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Physically active (according to the International Physical Activity Questionnaire (IPAQ)
- •Absence of any chronic condition that could preclude participation in a RT program or physical testing
- •Habitual protein consumption within RDA ≥ 0.8 ≤1.0 g.kg-
Exclusion Criteria
- •Prior history of anabolic steroids use, current or previous (≤ 3 m)
- •Use of ergogenic or protein-based supplements, current or previous (< 1 y)
- •Engagement in energy-restricted diets
Outcomes
Primary Outcomes
vastus lateralis muscle cross sectional area
Time Frame: Ultrasound images will be obtained both at baseline (before the comencement of the study) and immediately after the 12-week intervention period
the investigators used ultrasound measures of the vastus lateralis cross sectional area as the primary outcome
Secondary Outcomes
- lean mass(DXA scans will be taken both at baseline (before the comencement of the study) and immediately after the 12-week intervention period)
- fiber cross sectional area(Fiber cross sectional areas will be measured both at baseline (before the comencement of the study) and immediately after the 12-week intervention period)
- maximal isotonic strength(1-RM tests will be taken both at baseline (before the comencement of the study) and immediately after the 12-week intervention period)
Investigators
Hamilton Augusto Roschel da Silva
Doctor of Philosophy
University of Sao Paulo General Hospital