Modelling of human skin diseases using in vitro 3D reconstructed ski
Withdrawn
- Conditions
- 10014982inflammatory skin diseases (atopic dermatitis and psoriasis) and genodermatoses (skin diseases caused by genetic disorder)
- Registration Number
- NL-OMON46406
- Lead Sponsor
- Radboud Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Withdrawn
- Sex
- Not specified
- Target Recruitment
- 113
Inclusion Criteria
Patients that suffer from atopic dermatitis, psoriasis and genodermatoses
Exclusion Criteria
If it is likely that patients develop hypertrophic scars (patients are asked if they do/don't heal nicely after damage to the skin).
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>* The outcome measures of the cell culture experiments include: morphology of<br /><br>the epidermis and gene expression of the epidermal cells, following stimulation<br /><br>of the cells (e.g. by cytokines or microbes). Gene expression analysis will<br /><br>generate quantitative data (delta Ct of qPCR).<br /><br>* Histology will be scored semi quantitatively (stain intensity and<br /><br>localization).<br /><br>* We will identify relevant mutations of risk genes for psoriasis and AD. In<br /><br>case of genodermatoses, the causative genes are in general already known.<br /><br>* The effect of diagnosis and genotype on gene expression and histology will be<br /><br>analysed with multivariate techniques.</p><br>
- Secondary Outcome Measures
Name Time Method <p>None</p><br>