Prevention of Skeletal Muscle Adaptations to Traumatic Knee Injury and Surgery
Overview
- Phase
- Phase 1
- Intervention
- Not specified
- Conditions
- Anterior Cruciate Ligament Reconstruction
- Sponsor
- University of Vermont
- Enrollment
- 25
- Locations
- 1
- Primary Endpoint
- Cross-sectional Area of Skeletal Muscle Fibers (All Fibers)
- Status
- Completed
- Last Updated
- 5 years ago
Overview
Brief Summary
Traumatic knee injury is common and highly debilitating. Surgical reconstruction/repair improves knee biomechanics and function, but neuromuscular dysfunction persist for years despite rehabilitation, hindering resumption of normal activities, increasing risk of further injury and, in a majority of patients, hastening the development of knee osteoarthritis (OA). Our goal in this research study is to evaluate the utility of neuromuscular electrical stimulation (NMES), initiated following injury and maintained through the early post-surgical period, to prevent muscle atrophy and intrinsic contractile dysfunction compared to active control intervention of micro-electrical stimulation.
Investigators
Michael J. Toth, Ph.D.
Associate Professor of Medicine
University of Vermont
Eligibility Criteria
Inclusion Criteria
- •18-50 yrs
- •BMI \<35 kg/m2
- •acute, first-time, ACL rupture with or without meniscus injury
- •scheduled to undergo reconstruction with a BPTB autograft
Exclusion Criteria
- •history of prior knee/lower extremity surgery or non-surgical intervention (eg, intra-articular injection) on either leg
- •abnormal laxity of any lower extremity ligament other than the injured ACL
- •signs or symptoms of arthritis, autoimmune or inflammatory disease or diabetes
- •grade IIIb or greater articular cartilage lesions (ICRS criteria)
- •women who are/plan on becoming pregnant
Outcomes
Primary Outcomes
Cross-sectional Area of Skeletal Muscle Fibers (All Fibers)
Time Frame: Difference between injured and non-injured leg at 3 weeks post-surgery
Cross-sectional area of skeletal muscle fibers will be evaluated using immunohistochemistry, with specification of all relevant muscle fiber types
Maximal Single Muscle Fiber Shortening Velocity (Myosin Heavy Chain (MHC) IIA Fibers)
Time Frame: Difference between injured and non-injured leg at 3 weeks post-surgery
Maximal shortening velocity from segments of chemically-skinned single human muscle fibers will be assessed, with muscle fiber type determined post-measurement by gel electrophoresis
Cross-sectional Area of Skeletal Muscle Fibers (MHC IIA)
Time Frame: Difference between injured and non-injured leg at 3 weeks post-surgery
Cross-sectional area of skeletal muscle fibers will be evaluated using immunohistochemistry, with specification of all relevant muscle fiber types
Cross-sectional Area of Skeletal Muscle Fibers (Myosin Heavy Chain (MHC) I Fibers)
Time Frame: Difference between injured and non-injured leg at 3 weeks post-surgery
Cross-sectional area of skeletal muscle fibers will be evaluated using immunohistochemistry, with specification of all relevant muscle fiber types
Maximal Calcium-activated Tension Single Muscle Fiber Tension (Myosin Heavy Chain (MHC) IIA Fibers)
Time Frame: Difference between injured and non-injured leg at 3 weeks post-surgery
Tension (force per unit muscle fiber cross-sectional area) from segments of chemically-skinned single human muscle fibers will be assessed under maximal calcium-activated condition, with muscle fiber type determined post-measurement by gel electrophoresis
Secondary Outcomes
- Knee Extensor Peak Isokinetic Torque(Difference between injured and non-injured leg at 6 months post-surgery)