The Transcriptomic Study of Thai Patients With Atopic Dermatitis by Tape Strips
Trial Snapshot
- Phase
- Not Applicable
- Status
- Not yet recruiting
- Enrollment
- 100
- Locations
- 1
- Primary Endpoint
- Immune biomarkers
Study Overview
Brief Summary
This study will be use the tape strip technique to evaluate the skin biomarkers of atopic dermatitis among Thai patients to differentiate clinical phenotype.
Detailed Description
The patients will be enrolled in this study if they have been diagnosed with atopic dermatitis. All participants (AD patients and controls) will be evaluated their skin biomarkers by using tape stripping. The tape strips will be applied to the antecubital fossa to collect the epithelial samples. Then RNA was extracted from the tape strips for mRNA profiling to identify the immune and epidermal barrier genes.
Study Design
- Study Type
- Interventional
- Allocation
- Non Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 1 Year to 60 Years (Child, Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Children (age 1-18 years old) with mild atopic dermatitis
- •Children (age 1-18 years old) with moderate to severe atopic dermatitis
- •Children (age 1-18 years old) with moderate to severe atopic dermatitis and food allergy
- •Adult (age 18-60 years old) with atopic dermatitis
- •Healthy individuals (1-60 years old)
- •Patients with asthma (1-60 years old)
Exclusion Criteria
- •Active skin infections
- •Used systemic immunosuppressants within 4 weeks
- •Used topical steroids or immunomodulators within 1 week
- •Used moisturizers within 12 hours before evaluation
Arms & Interventions
Children with mild atopic dermatitis
- age 1-8 years old
- mild degree of atopic dermatitis
Intervention: Tape strips (Genetic)
Children with moderate-severe atopic dermatitis
- age 1-8 years old
- moderate or severe degree of atopic dermatitis
Intervention: Tape strips (Genetic)
Children with food allergy and moderate-severe atopic dermatitis
- age 1-8 years old
- moderate or severe degree of atopic dermatitis
- IgE mediated food allergy
Intervention: Tape strips (Genetic)
Adult with atopic dermatitis
- age 18-60 years old
- mild-severe atopic dermatitis
Intervention: Tape strips (Genetic)
Healthy
- age 1-60 years old
- no history of atopic diseases
Intervention: Tape strips (Genetic)
Healthy with Asthma
- age 1-8 years old
- doctor diagnosed asthma
- no history of chronic or chronic relapsing eczema
Intervention: Tape strips (Genetic)
Outcomes
Primary Outcomes
Immune biomarkers
Time Frame: 1 month
Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0
Cellular AD biomarkers
Time Frame: 1 month
Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0
Barrier biomarkers
Time Frame: 1 month
chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0
Secondary Outcomes
No secondary outcomes reported
Investigators
Tassalapa Daengsuwan
Assistant Professor
Queen Sirikit National Institute of Child Health