Effect of diode laser application as an adjunct to non-surgical periodontal therapy on the reduction of red complex microorganisms in type 2 diabetic patients.

It will be a split mouth longitudinal  clinical study involving subjects with periodontal pocket depth 5-6 mm atleat two teeth.Subjects will receive full mouth ultrasonic supragingival scaling at first visit .The clinical parameters ,gingival index, plaque index ,probing pocket depth and clinical attachment level will be assessed at baseline ,2 weeks and 3 months. The sample collection for microbiological analysis will be done at 2 weeks and 3 months..After 2 weeks of recall ,plaque sample collection and reassessment of the clinical parameters will be done . A full mouth subgingival scaling followed by diode laser application on the test site ll be done in the same visit. The patient is recalled at the end of 3 months the clinical parameters are reassessed and plaque samples are again collected for microbiological analysis. The red complex bacterial count will  be estimated for both study group using RT-PCR.