Effect of blackcurrant consumption on physical fitness, bioenergetics and natural immunity during high intensity training; involvement of mitochondrial adaptation?
- Conditions
- Fitness adaptation, bioenergetics and mitochondria number/mass, function and fagility.Fitness adaptation and natural immunityInflammatory and Immune System - Normal development and function of the immune system
- Registration Number
- ACTRN12614000019606
- Lead Sponsor
- The New Zealand Institute of Plant and Food Research
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ot yet recruiting
- Sex
- All
- Target Recruitment
- 10
Healthy persons of either gender
Participants in this study will be excluded if they have any of the following conditions: (i) blood borne diseases, e.g. hepatitis; (ii) clinically diagnosed high/low blood pressure; (iii) pregnant, or planning to get pregnant; (iv) recent bacterial or viral illness or vaccinations; (v) chronic illness that affects their immune system (e.g. lupus) or (vi) any medication that affects the properties of blood, e.g. clotting.
In addition subjects recruited for this study will be excluded if they have (i) any health conditions that affect their ability to perform the exercise regime required in this study (i.e. heart and/or breathing problems, hernia or injury) or (ii) intolerance, hypersensitivity or allergy to foods, especially fruit.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Changes in fitness, biogenerensis and immunity before and after HIT training. <br>(1) Exercise (physical and subject) parameters: VO2max L/min, O2/CO2 exchange, Heart rate, Work output, Lactate threshold, rating of perceived exertion (RPE), Fatigue, pain/soreness VAS & thigh swelling. <br>(2) Innate immunity. Circulating inflammatory markers (IL-6, IL-10, C-reactive protein), leukocyte immune responsiveness to bacterial (TLR4 /TL2 ligands) and viral (TLR7 ligands) antigens (ex vivo studies) <br>(3) Mitochondrial number, function (respirometry)and fagility status in circulating leukocytes and platelets.<br>[The above assessment will be taken as part of the 15K cycle time-trial fitness assessment. Measruements will be be made before and immediately after the completion of the 15Km time trial and then after 20 mins recovery.<br>]
- Secondary Outcome Measures
Name Time Method Changes in blood biochemistry (as indicators of change in general health and well-being). Antioxidant (FRAP, SOD, catalase, GPx), oxidative stress parameters (carbonyls, MDA) and insulin and free fatty acid profiles.[The above assessment will be taken as part of the 15K cycle time-trial fitness assessment. Measruements will be be made before and immediately after the completion of the time trial and then after 20 mins recovery.]