Cocoa catabolites
Not Applicable
- Conditions
- ot applicable. The aim is to establish the catabolic and absorption profile of cocoa flavanols catabolites generated by the human colonic microbiota.Healthy Volunteers
- Registration Number
- RPCEC00000389
- Lead Sponsor
- Compañía Nacional de Chocolates SAS
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Pending
- Sex
- All
- Target Recruitment
- 8
Inclusion Criteria
1.Male and female,
2. between 18 and 50 years old,
3. habitual consumers of products derived from cocoa such as chocolate, cocoa, among others.
4. Non-smokers.
5. No diagnosis of gastrointestinal diseases.
6. No consumption of antibiotics, antiparasitic, and/or laxatives in the last three months.
7. Non-vegetarian.
8. Not be enrolled in weight loss programs or consuming weight loss supplements.
Exclusion Criteria
1. Pregnant or breastfeeding women.
2. High-performance athletes.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method 1. Catabolites in stool samples (gas chromatography/mass spectrometry; colorimetric methods). Measurement time: baseline and 5 days after chocolate consumption.<br>2. Catabolites in urine samples (gas chromatography/mass spectrometry; colorimetric methods). Measurement time: baseline (occasional urine first in the morning), 10 hours after the first consumption, and 5 days after the consumption of chocolate.<br>3. In vitro, microbial catabolism will be evaluated on a fraction of flavanols (gas chromatography/mass spectrometry; colorimetric methods). Measurement time: 0, 3, and 6 hours.
- Secondary Outcome Measures
Name Time Method Composition of the microbiota (Operational Taxonomic Units, defined to 97% similarity of DNA sequences of variable regions of the 16S rRNA gene). Measurement time: baseline and 5 days after chocolate consumption.