To Study the role of immature platelet fraction in children with severe thrombocytopenia

The assessment of bone marrow thrombopoietic activity in patients with thrombocytopenia is necessary to achieve an accurate diagnosis and administer effective Treatment.Immature platelets can be useful marker of thrombopoietic activity.They represent the most recently produced platelets released into the circulation by regenerated bone marrow megakaryocytes.The number of circulating immature platelets reflects the rate of thrombopoiesis and bone marrow function and, as such, may be useful in the determination of thrombocytopenic etiology.A study done in UK in the year 2019 showed the IPF was significantly higher in cases of increased platelet consumption compared with controls. Another study in Republic of Korea between January 2016 to July 2018 showed IPF is higher in hyper destructive thrombocytopenia compared to control group. In this study the blood sample collected during admission for evaluation purpose will be used for estimation of immature platelet fraction in all the children included in the study. The sample will be processed at the earliest and processed by flowcytometry using optical fluorescence method. The data collected at the end of the study will be used to study the role of immature platelet fraction in different aetiologies of severe thrombocytopenia.