Exploratory Study of Cellular Reservoirs in Blood From HIV Infected Patients

Not Applicable

Completed

• Conditions: HIV Infection
• Interventions: Other: additional blood sample

• Registration Number: NCT02858414
• Lead Sponsor: Centre Hospitalier Universitaire de Besancon

Brief Summary

This study evaluates the impact of highly active antiretroviral therapy on the size of the latent viral reservoirs in resting CD4+ T cells and monocytes in HIV positive patients. The activation state of the cells will be assessed, by measuring the activation of Akt, to determine its influence on the size of the viral reservoirs.

Detailed Description

Not available

Study Timeline

• Study Start: 2015-04-02
• Study First Submitted: 2016-07-29
• Study First Submitted QC: 2016-08-03
• Study First Posted: 2016-08-08
• Primary Completion: 2017-10-04
• Study Completion: 2017-10-04
• Status Verified: 2018-12
• Last Update Submitted: 2018-12-05
• Last Update Posted: 2018-12-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 41

Inclusion Criteria

• HIV infected patients under Highly Active Anti-Retroviral Therapy (HAART) or Naïve from treatment

Exclusion Criteria

• Age limits
• Pregnancy
• Legal restrictions to consent

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
blood sample	additional blood sample	-

Primary Outcome Measures

Name	Time	Method
Measure of the integrated viral reservoir size	day 1	Measure by Alu-PCR. Results are given in number of integrated proviral DNA copies per 1 million cells.
Measure of the proviral reservoir size	day 1	Measure by Total Proviral HIV DNA detection kit. Results are given in number of total proviral DNA copies per 1 million cells.

Secondary Outcome Measures

Name	Time	Method
Measure of Akt activation	day 1	Measure of Akt activation in live cells by flow cytometry

Trial Locations

Locations (1)

Centre Hospitalier Régional Universitaire; 🇫🇷 Besançon, France