Ex Vivo Study of the Role of Soluble Endothelial Protein C Receptor in Cirrhosis-associated Hypercoagulability State
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 300
- 试验地点
- 2
- 主要终点
- Thrombin generation assay (area under the curve/ Endogenous Thrombin Potential) with thrombomodulin.
研究概览
简要总结
Cirrhosis is a condition in which the liver does not function properly due to long-term damage. This damage is characterized by the replacement of normal liver tissue by scar tissue.
The liver carries out several necessary functions, including synthesis of proteins participating in blood coagulation process. Some of these proteins contribute to coagulation and others make blood more fluid. In healthy people there is a balance between the two.
In cirrhotic patient, there is an imbalance inducing hypercoagulation (hypercoagulability state). Cirrhotic patients are so known to be at risk of vein thrombosis (for example portal vein thrombosis: clot in hepatic vein).
Mechanisms leading to this imbalance are unclear. Studies need to be completed to improve patient's management.
The EPCRs (Endothelial Protein C Receptor soluble) takes part in blood coagulation process. Previous studies have shown that blood levels of EPCRs are increased in patients with cirrhosis.
The primary purpose of the study is to evaluate if the EPCRs could play a role in cirrhosis-associated hypercoagulability state.
详细描述
Three groups will be constitued: one with cirrhotic patients, one with healthy volunteers, and one with patients with hepatocellular carcinoma, a form of liver cancer (previous studies have also shown that blood levels of EPCRs are increased in these patients).
All participants will undergo a unique visit at hospital. An informed consent will be obtained before any related study procedures*.
* procedures: physical examination (including height, weight, waist measurement), vital signs (blood pressure, heart rate, temperature), medical history, concomitant medication, blood sample.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Screening
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Patients with cirrhosis
- •Inclusion criteria :
- •Male, age 18 or older
- •Cirrhosis, defined by: prothombin time ≤ 70% +/- liver dysmorphism +/- Fibroscan > 12.5 kPa +/- histology +/- portal hypertension associated with hepatocellular insufficiency
- •Cirrhosis associated with hepatitis B or C, or alcoholic liver disease, or non alcoholic steato hepatitis
- •Measurable portal flow by ultrasound doppler
- •Child Pugh score A,B or C, without acute infection or acute bleeding
- •Signed written informed consent
- •Affiliation to french social security system
排除标准
- •Known history of vein thrombosis
- •Known family history (in first-degree relative) of spontaneous thrombosis
- •Partial or complete portal system vein thrombosis
- •Uncontrolled infection after 7-day course of antibiotics
- •Anticoagulant intake
- •Hepatocellular carcinoma
- •Protected or deprived of liberty adult
- •Any medical or surgical history that could interfere with the study, as judged by the investigator
- •Blood transfusion within 7 days
- •Participation in a clinical trial for drug
- •Healthy volunteers
- •Inclusion criteria :
- •Male, age 18 or older
- •Body Mass Index between 18 and 30 kg/m2
- •Normal physical examination
- •Willing and able to comply with requirements of the study
- •Agree to be registered in the french national registry for healthy volunteers
- •Signed written informed consent
- •Affiliation to french social security system
- •Exclusion criteria :
- •Any medical or surgical history that could interfere with the study, as judged by the investigator
- •Participation in a clinical trial for drug
- •Protected or deprived of liberty adult
- •Known history of vein thrombosis
- •Known family history (in first-degree relative) of spontaneous thrombosis
- •Anticoagulant intake
- •Patients with hepatocellular carcinoma (HCC)
- •Inclusion criteria :
- •Male, age 18 or older
- •Histological diagnosis of HCC, or use of BCLC criteria for diagnosis
- •Treatment naïve HCC
- •Measurable portal flow by ultrasound doppler
- •For patients with cirrhosis: Child Pugh score A,B or C, without acute infection or acute bleeding
- •Signed written informed consent
- •Affiliation to french social security system
- •Exclusion criteria :
- •Known history of vein thrombosis
- •Known family history (in first-degree relative) of spontaneous thrombosis
- •Partial or complete portal system vein thrombosis
- •For patients with cirrhosis: uncontrolled infection after 7-day course of antibiotics
- •Anticoagulant intake
- •Protected or deprived of liberty adult
- •Any medical or surgical history that could interfere with the study, as judged by the investigator
- •Blood transfusion within 7 days
- •Prior treated malignancy within the previous 2 years (except basal cell and squamous cell skin cancer, and superficial bladder cancer)
- •Participation in a clinical trial for drug
结局指标
主要结局
Thrombin generation assay (area under the curve/ Endogenous Thrombin Potential) with thrombomodulin.
时间窗: 24 months
次要结局
- Thrombin generation assay parameters : time to peak(24 months)
- Thrombin generation assay parameters : peak height(24 months)
- Thrombin generation assay parameters : lag time(24 months)