Assessment Of Gene Expression Of Suppressor Of Cytokine 3, Interferons Alpha And Beta In Cutaneous Warts
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- This study aims to assess the expression of genes of IFN-β in cutaneous warts.
研究概览
简要总结
Warts are common epidermal growths caused by various strains of human papilloma virus (HPV). Viral warts are common with a prevalence rate of 7-12%.
Human papilloma virus is small non enveloped viruses that contain a double-stranded DNA genome. There are more than 200 HPV genotypes are reported, however, the high-risk types, including HPV genotype-16 (HPV16), 18, 31, and 45, are the causes of 80% of cervical cancers.
详细描述
Cutaneous warts are benign lesions caused by low risk-HPV. Of which the most prevalent genotypes are HPV1/2/3/4/10/27/57, and from these genotypes 1, 2, 4, 27, 57 cause common warts. Palmoplantar warts can be caused by genotypes 1 and 2. Condyloma acuminata (anogenital warts) are common sexually transmitted infections caused primarily by HPV-6 and HPV-11 (in 90% of cases) and occasionally by HPV-16 and HPV-18.
About 90% of HPV infections are eliminated within two years due to an efficient immune response. In the form of correct activation of the elements of the innate immune response, such as macrophages, polymorphonuclear cells, natural killer (NK) cells, which release immunomodulatory molecules that help control the infection. In addition, HPV-infected keratinocytes may act as non-professional antigen-presenting cells to promote the clearance of infected cells through the secretion of antiviral and pro-inflammatory mediators.
Interferon (IFN) belongs to a family of inducible cytokines which promote an"antiviral state" in infected cells and neighboring cells through the activation of interferon-stimulated genes (ISG). Three IFN types have been identified (type I, II and III), of which I and III are involved in the innate immune response. Type I IFNs include IFN-α, IFN-β, IFN-ɛ, IFN-κ and IFN-ω.
Most type I IFNs are induced through the binding of viral products to pattern recognition receptors, leading to activation of interferon response factors to drive the synthesis of IFN molecules. After secretion, type I IFNs bind to its receptor, which induces the phosphorylation of signal transducer and activator of transcription 1 (STAT1) and STAT2, which then translocate to the nucleus to induce transcription of (ISGs).
The antiviral effects of IFN-I are mediated by inducing several (ISGs) which impair viral replication through inhibition of protein translation and degradation of viral RNA. ISGs also activate the survival of innate and adaptive immune cells including dendritic cells, macrophages, NK cells and T cells. So, IFN-I helps to control the infections.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •The study will include patients with cutaneous warts of various types aged 18-50 years old.
排除标准
- •Patient on anti-inflammatory and antioxidant drugs
- •Skin infections, other than warts
- •Skin diseases
- •Systemic infection.
- •Malignancies
结局指标
主要结局
This study aims to assess the expression of genes of IFN-β in cutaneous warts.
时间窗: from April 2023 to December 2023
Under complete sterile precautions, Skin biopsy will be taken from healthy volunteers of the control group via 2 mm disposable punches and from patients with wart lesion. The specimen will be put in sterile plane tube containing saline and will be transferred immediately to the central research laboratory to be processed as follow: 1. Tissue grinding into smaller pieces then will be subjected for RNA extraction by automated kits according to its manufacture instruction. 2. Conversion of RNA to double stranded DNA using RT( reverse transcriptase conversion Kits ) according to its manufacture instruction using conventional PCR. 3. Detection of expression of IFN-β gene in each samples using Real-Time PCR
This study aims to assess the expression of genes of SOCS3 in cutaneous warts.
时间窗: from april 2023 to December 2023
Under complete sterile precautions, Skin biopsy will be taken from healthy volunteers of the control group via 2 mm disposable punches and from patients with wart lesion. The specimen will be put in sterile plane tube containing saline and will be transferred immediately to the central research laboratory to be processed as follow: 1. Tissue grinding into smaller pieces then will be subjected for RNA extraction by automated kits according to its manufacture instruction. 2. Conversion of RNA to double stranded DNA using RT( reverse transcriptase conversion Kits ) according to its manufacture instruction using conventional PCR. 3. Detection of expression of SOCS3 gene in each samples using Real-Time PCR
This study aims to assess the expression of genes of IFN-α in cutaneous warts.
时间窗: from April 2023 to December 2023
Under complete sterile precautions, Skin biopsy will be taken from healthy volunteers of the control group via 2 mm disposable punches and from patients with wart lesion. The specimen will be put in sterile plane tube containing saline and will be transferred immediately to the central research laboratory to be processed as follow: 1. Tissue grinding into smaller pieces then will be subjected for RNA extraction by automated kits according to its manufacture instruction. 2. Conversion of RNA to double stranded DNA using RT( reverse transcriptase conversion Kits ) according to its manufacture instruction using conventional PCR. 3. Detection of expression of IFN-α gene in each samples using Real-Time PCR
次要结局
未报告次要终点
研究者
Reham Helal Aboelhamd
principal investigator
Sohag University