Influence of Polymorphysms in the Fto and Ppar Gen Genes, Systemic Inflammation and Oxidative Stress in the Magnitude of Weight Loss Induced by Intermittent or Moderate Continuous High Intensity Training Programs
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 57
- 主要终点
- Body Composition. The changes are being evaluated.
研究概览
简要总结
The study focuses on the influence of polymorphism in the FTO genes rs9939609 and PPARᵧ Pro12Ala, oxidative stress and systemic inflammation on changes in body composition and rest metabolism induced by HIIT and continuous aerobic programs in obese or overweight individuals.
详细描述
Although the positive effects of continuous aerobic training are already well documented in the literature on health aspects. The weight loss induced by physical training is still very discrete when compared to other antiobesity means. Studies demonstrate an important variability in responses to physical exercise, indicating that individuals respond more or less to weight loss. Among these aspects, the factors that may influence exercise-induced weight loss have not yet been elucidated. Thus, the objective of the study is to analyze the influence of polymorphism in the genes FTO rs9939609 and PPARᵧ Pro12Ala, oxidative stress and systemic inflammation on changes in body composition and rest metabolism induced by continuous and continuous aerobic programs. Healthy individuals with overweight and / or obesity, aged 20-45 years, were randomly assigned to three groups: Continuous Aerobic (AC), Intermittent High Intensity Training (HIIT) and Control Group (CG). Groups (AC) and (HIIT) engaged in a 12-week program. Before the start of the program, at the sixth week and 48 hours after the intervention, they performed blood collections, ergospirometry, dual X-ray densitometry (DEXA), nutritional assessments. In addition, collection of buccal mucosa was performed for genotyping of polymorphisms studied. Initial differences between the groups, as well as between the dependent variables studied will be assessed using the two-way ANOVA test or its non-parametric equivalent. Correlation and regression tests will be performed to verify the influence of the independent variables and the magnitude of the exercise-induced weight loss, according to the normality of the data or differences between the standard deviations. The hypothesis tested is that genetic and physiological factors influence the weight loss independent of the training modality.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Men and women aged 20-45 years;
- •Were insufficiently active for at least six months prior to recruitment as determined by the International Physical Activity Questionnaire (IPAQ) (<150 minutes of moderate to intense physical activity / week) (MATSUDO, 2001);
- •Were overweight or obese (BMI between 25kg / m2 and 39.9kg / m2) (WHO, 2015);
- •Did not suffer any change in weight (more than 5kg in the last three months);
- •Do not be smokers or acololistas (maximum of 2 doses / day);
- •They may not be users of supplements and / or medications that influence weight loss or weight gain;
- •No history of coronary, respiratory, metabolic, or musculoskeletal disease affecting ability to participate in the training program;
- •Have been considered fit in an exercise test performed immediately prior to physical intervention;
- •Women are not menopausal (menstrual cycle stopped more than one year) or have symptoms related to the climacteric phase.
排除标准
- •Individuals who lack at least 25% of the total training program or two consecutive weeks;
- •Initiate medication treatment, supplementation, diet and / or other physical training program during the intervention period;
- •During training, osseous and / or myoarticular lesions that affect the ability to continue participating in the intervention.
结局指标
主要结局
Body Composition. The changes are being evaluated.
时间窗: Before the intervention protocol and 48 hours immediately after the last exercise session.
The procedure used for analysis is done using a Dual Energy Radiological Absortiometry (DEXA) equipment. The measurement of the body fat and fat free mass percentage measure is obtained by means of a full body scan using the LUNAR PRODIGY DF + 14.319 Radiation (Madison, WI) brand device, following manufacturer's protocols. The body mass is evaluated by means of a balance (Sanny®, São Bernardo do Campo - São Paulo, Brazil), with the volunteer barefoot and in orthostatic position using a Toledo scale sensitive to 100 g. The stature is evaluated by a stadiometer with a tape calibrated at 0.1 of the same mark. Waist circumference and other body perimeters are measured with a 0.1 cm Anthropometric Tape (Sanny®, São Bernardo do Campo - São Paulo, Brazil). Weight and height data are used to calculate BMI using the equation adopted by the WHO: BMI = (Weight / (Stature) 2).
次要结局
- Metabolic Rate of Rest. The changes are being evaluated.(Before the intervention protocol and 48 hours immediately after the last exercise session.)
- Lipid and Glycemic Profile. The changes are being evaluated.(The collections will be done 24 hours before, in the 6th week and 48 hours after the end of the intervention.)
- Systemic Inflammation (Analysis of alpha-1-glycoprotein acid). The changes are being evaluated.(The collections will be done 24 hours before, in the 6th week and 48 hours after the end of the intervention.)
- DNA Extraction and Genotyping(The genetic collection will be made in the 6th week of the intervention.)
- Oxidative stress (Malondialdehyde). The changes are being evaluated.(The collections will be done 24 hours before, in the 6th week and 48 hours after the end of the intervention.)
- Oxidative stress (Total antioxidant capacity). The changes are being evaluated.(The collections will be done 24 hours before, in the 6th week and 48 hours after the end of the intervention.)
- Systemic Inflammation (Plasma ultra-sensitive C-reactive protein). The changes are being evaluated.(The collections will be done 24 hours before, in the 6th week and 48 hours after the end of the intervention.)
研究者
Raquel Suelen Brito da Silva
Master/ Principal Investigator
Federal University of Paraíba