Acid Production in Dental Plaque After Exposure to Probiotic Bacteria

Not Applicable

Completed

• Conditions: Caries
• Interventions: Dietary Supplement: Probiotic Lactobacilli reuteri; Dietary Supplement: Placebo

• Registration Number: NCT01700712
• Lead Sponsor: University of Copenhagen

Brief Summary

The increasing interest in probiotic lactobacilli in health maintenance has raised the question of potential risks. One possible side effect could be an increased acidogenicity in dental plaque. The aim of this study was to investigate the effect of probiotic lactobacilli on plaque lactic acid (LA) production in vitro and in vivo.

Methods. subjects (n=18) were given lozenges with probiotic lactobacilli (L. reuteri DSM 17938 and ATCC PTA 5289) or placebo for two weeks in a double-blinded, randomized cross-over trial. The concentration of LA in supragingival plaque samples was determined at baseline and after 2 weeks. Salivary counts of mutans streptococci (MS) and lactobacilli were estimated with chair-side methods.

Detailed Description

Not available

Study Timeline

• Study Start: 2010-12
• Primary Completion: 2011-10
• Study Completion: 2011-10
• Status Verified: 2012-10
• Study First Submitted: 2012-10-02
• Study First Submitted QC: 2012-10-03
• Last Update Submitted: 2012-10-03
• Study First Posted: 2012-10-04
• Last Update Posted: 2012-10-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

• moderate to high counts of salivary mutans streptococci (>104 CFU) as estimated with the Dentocult SM chair-side test,
• no visible open caries lesions or periodontal disease,

Exclusion Criteria

• smokers,
• ingestion of probiotic bacteria within the last two months,
• ingestion of antibiotics within the last two months

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
L. reuteri (DSM 17938 and ATCC PTA 5289; 1x108 CFU	Probiotic Lactobacilli reuteri	2 tablets a day for 2 weeks
Sugar pill	Placebo	2 tablets a day for 2 weeks

Primary Outcome Measures

Name	Time	Method
Acid production in plaque after exposure to probiotic bacteria	2 weeks

Secondary Outcome Measures

Name	Time	Method
Acid producton in plaque after in vitro exposure to probiotic bacteria	1 hour

Trial Locations

Locations (1)

University of Copenhagen; 🇩🇰 Copenhagen, Denmark