Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 57
- 试验地点
- 4
- 主要终点
- Percentage of sperm motility
研究概览
简要总结
The goal of this clinical trial is to compare the effect of apigenin supplementation in freezing medium with no apigenin supplementation in freezing medium on post-thaw human sperm motility. It will also evaluate the sperm viability, total antioxidant capacity, and oxidative stress markers. The main questions it aims to answer are:
- Does apigenin supplementation in freezing medium improve the post-thaw sperm motility?
- Does apigenin supplementation in freezing medium improve the post-thaw sperm viability, total antioxidant capacity, and oxidative stress?
详细描述
All normal semen samples from participants who give written informed consent and meet the eligibility requirements will be included in the study. Prepared semen samples were divided into 3 groups.
- Pre-freezing sperm suspension
- Frozen-thawed control without apigenin supplementation in freezing medium
- Frozen-thawed sperm suspension with apigenin 0.2 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension. The last 2 groups will be stored in liquid nitrogen for at least one week. After thawing, the sperm suspensions will be evaluated for motility, viability, total antioxidant capacity, and oxidative stress markers.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Supportive Care
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy men aged 18-45 years who can receive semen analysis services at the Reproductive Biology Unit, Chulalongkorn Hospital, Thai Red Cross Society
- •Normal semen analysis results according to the World Health Organization criteria (Volume ≥ 1.4 mL, Sperm concentration ≥ 16 million/mL, Total motility ≥ 42%, Progressive motility ≥ 30%)
- •Giving consent
排除标准
- •A history of accidents, radiation to the testes, or a history of male reproductive system surgery
- •Undescended testis
- •Varicocele
- •A history of taking certain medications that affect sperm production, such as chemotherapy, hormones
- •Currently having sexually transmitted disease
结局指标
主要结局
Percentage of sperm motility
时间窗: At least 1 week of freezing
Sperm motility (total motility, progressive motility, non-progressive motility, immotile spermatozoa) will be reported in percentage. Compare results among freezing medium with apigenin group, control group, and baseline pre-freeze sperm suspension.
次要结局
- Percentage of sperm viability comparison between intervention and control group(At least 1 week of freezing)