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Dupilumab Efficacy on Bronchial Inflammation, Small Airways Disfunction and Mucous Secretion

Recruiting
Conditions
Asthma; Eosinophilic
Registration Number
NCT05787678
Lead Sponsor
Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau
Brief Summary

Unicentric prospective real life study designed to analyse the efficacy of Dupilumab to reduce bronchial inflammation of asthma measured by inflammatory cell counts obtained in induced sputum and other related characteristics (small airway dysfunction and mucin production) in uncontrolled severe asthma patients in the real life setting without control group

Detailed Description

After patients signed informed consent, before (visit 1), after 4-5 months (visit 2), and after 12 months (visit 3) Dupilumab treatment began, the following techniques and measurements will be performed or collected: demographic data, blood analyses (eosinophil counts and total IgE), skin prick-test to common aeroallergens (only in the visit 1), asthma control test (ACT), sino-nasal outcome test (SNOT-22), test of adherence to inhalers (TAI), number of severe asthma exacerbations (in the previous year; in the visit 2, in the previous 4 months), oral and inhaled steroid daily doses received, spirometry, exhaled nitric oxide (FeNO), pulmonary volumes, carbon monoxide transference, oscillometry, and induced sputum (inflammatory cell counts and mucin profile \[MUC1, MUC2, MUC5AC, MUC5B\]). All patients will be performed at visits 1 and 3 in a 3D culture of primary nasal epithelial cells from asthmatic patients the pattern of gene and to identify new soluble biomarkers through quantitative proteomic analysis of culture supernatants.

Also a chest CT scan with expiratory images will be done only in visits 1 and 3.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
30
Inclusion Criteria
  • 18 -80 years
  • Severe uncontrolled eosinophilic asthma by GINA (Global Initiative for Asthma) criteria
  • Clinical indication of Dupilumab
Exclusion Criteria
  • Smoking over 10 pack/year
  • Treatment with monoclonal antibodies treatment during the previous three months
  • Refuse to sign informed consent

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Number of bronchial inflammatory cells1 year

Count of induced sputum leucocytes

Secondary Outcome Measures
NameTimeMethod
Mucin production from epithelial cells1 year

Levels of MUC 1, MUC2, MUC5AC, and MUC5B

Trial Locations

Locations (1)

Hospital de la Santa Creu i Sant Pau. Carrer Mas Casanovas 90.

🇪🇸

Barcelona, Spain

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