Clinical Study of Redirected Autologous T Cells With a Chimeric Antigen Receptor in Patients With Malignant Tumors
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 18
- 试验地点
- 1
- 主要终点
- Number of participants with CRA T-related adverse events as assessed by CTCAE v4.03
研究概览
简要总结
A single arm, open-label pilot study is designed to determine the safety, efficacy and cytokinetics of CAR T cells in patients with malignant tumors with positive antigen targets.
CAR T cells are genetically engineered to express single-chain variable fragment (scFv) targeting indication-specific antigens.
The investigational CAR T cells and proposed indications are as follows:
CAR-CD19 T cells for B cell leukaemia/lymphoma; CAR-BCMA T cells for myeloma; CAR-GPC3 T cell for hepatocellular carcinoma; CAR-CLD18 T cells for pancreatic carcinoma and adenocarcinoma of esophagogastric junction.
详细描述
This study is designed to determine the safety, tolerability and engraftment potential of lentivirus-transduced CAR T cells in patients with malignant tumors.
Primary objectives:
- Determine the safety and tolerability of CAR T cells (autologous T cells transduced with chimeric antigen receptors recognizing CD19, BCMA, GPC3 and Claudin18.2) in patients with malignant tumors (B-cell lymphoblastic leukaemia/lymphoma, myeloma, hepatocellular carcinoma, pancreatic carcinoma and adenocarcinoma of esophagogastric junction).
- Observe the cytokinetics of CAR T cells.
Secondary objectives:
- Observe the efficacy of CAR T cells in patients with malignant tumors.
- Make an evaluation on the distribution and in vivo survival of CAR T cells in targeted tissues.
- Observe the immunogenicity of CAR T cells, and determine if there are anti-scFv cellular immune response and anti-scFv humoral immune response.
- Observe the changes of cell subsets for CAR T cells against T cells (Tcm, central memory T lymphocytes; Tem, effector memory T lymphocytes; Treg, regulatory T-lymphocytes).
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •I. B-Cell Lymphoblastic Leukaemia/Lymphoma
- •Patients aged between 18 ~ 65 with B-cell lymphoblastic leukaemia/lymphoma.
- •CD19-positive B-cell lymphoblastic leukaemia/lymphoma.
- •Patients with unmet medical needs for which there are no effective therapies known at this time:
- •A. Relapsed or Refractory (r/r) Acute Lymphoblastic Leukemia (ALL)
- •Patients with r/r ALL for whom hematopoietic stem cell transplantation (HSCT) is not suitable due to following reasons:
- •Excessive tumor burden or concomitant disease;
- •No donor available.
- •B. CD19-positive Follicular Lymphoma:
- •At least 2 prior chemotherapy regimens (not including single agent monoclonal antibody (Rituxan) therapy;
- •Less than 6 months between last chemotherapy and disease progression (most recent progression free interval < 6 months);
- •Disease progression after most recent systemic therapy (chemotherapy, MoAb, etc.).
- •C. Chronic Lymphocytic Leukemia (CLL)
- •At least 2 prior chemotherapy regimens (not including single agent monoclonal antibody (Rituxan) therapy);
- •Less than6 months between last chemotherapy and disease progression (most recent progression free interval < 6 months);
- •Not eligible or appropriate for conventional HSCT.
- •Disease progression after most recent systemic therapy (chemotherapy, MoAb, etc.).
- •D. Mantle Cell Lymphoma
- •At least 2 prior chemotherapy regimens (not including single agent monoclonal antibody (Rituxan) therapy);
- •Disease progression after most recent systemic therapy (chemotherapy, MoAb, etc.)；
- •Relapsed after prior autologous SCT.
- •E. B-Cell Prolymphocytic Leukemia (PLL)
- •Relapsed or residual disease after at least 1 prior therapy and not eligible for HSCT.
- •F. CD19-positive Diffuse Large B Cell Lymphoma
- •At least 2 prior chemotherapy regimens (not including single agent monoclonal antibody (Rituxan) therapy;
- •Stage III-IV disease.
- •Less than 6 months between last chemotherapy and disease progression (most recent progression free interval < 6 months);
- •Disease progression after most recent therapy (chemotherapy, MoAb, etc.).
- •Expected survival > 12 weeks.
- •At least one measurable lesion (≥ 10 mm) for patients with lymphoma.
- •ECOG scores 0-1, or KPS scores >
- •Adequate venous access for apheresis or venous sampling, and no other contraindications for leukapheresis.
- •WBC ≥ 2.5×10^9/L; PLT ≥ 60×10^9/L (for patients with lymphoma); Hb ≥ 9.0 g/dL; LY ≥ 0.47×10^9/L; LY% ≥ 15%.
- •Serum Alb ≥ 30 g/L.
- •Serum creatinine ≤ 1.5 ULN.
- •ALT ≤ 2.5 ULN; AST ≤ 2.5 ULN.
- •Serum total bilirubin ≤ 1.5 ULN. The above lab results should not include those obtained from continuous supportive treatment that is ongoing.
- •II. Myeloma
- •Patients aged between 18 ~ 75 with relapsed or refractory multiple myeloma.
- •Bone marrow sample is confirmed as BCMA-positive by flow cytometry or pathological examination.
- •Patients with relapsed or refractory malignancies who meet the following descriptions:
- •Curative efficacy is little or disease progressed after 2 courses of standard treatment regimen;
- •Disease relapsed after chemotherapy or HSCT. Curative efficacy is little or disease progressed after 2 courses of original treatment regimen;
- •More than 30 days between last treatment and disease progression;
- •There is no indication for HSCT at present;
- •Disease progression is defined as per "Chinese Guidelines for Diagnosis and Treatment of Multiple Myeloma (Version 2015)". One or more of the following conditions should be met:
- •i. Serum M-protein increases ≥ 25% (absolute increase should be ≥ 5 g/L). If serum M protein is ≥ 50 g/L at baseline, increase of serum M protein can be ≥ 10 g/L; ii. Urine M-protein increases ≥ 25% (absolute increase should be ≥ 200 mg/24 h); iii. If the serum and urine M-protein are not detectable, a ≥ 25% increase in the difference between involved and uninvolved FLC levels is required (absolute increase should be ≥ 100 mg/L); iv. Bone marrow plasma cell percentage increases ≥ 25% (absolute increase should be ≥ 10%); v. Size of existing bone lesions or soft tissue plasmacytomas increased by ≥ 25%, or development of new lytic bone lesions or oft tissue plasmacytomas; vi. Development of hypercalcemia that can be attributed to plasma cell proliferative disorder (corrected calcium is > 2.8 mmol/L or 11.5 mg/dL).
- •Expected survival > 12 weeks.
- •Disease is measurable, and at least one of the following conditions should be satisfied:
- •Serum M-protein is ≥ 10 g/L;
- 另有 40 项未显示
排除标准
- •Patients with any of the following conditions are not eligible for this study.
- •Transduction of target lymphocytes < 10%, expansion in response to αCD3/CD28 costimulation < 5-fold.
- •Pregnant or lactating women.
- •HIV positive, or HCV positive
- •Uncontrolled active infection, including active tuberculosis and HBV DNA copies ≥ 1×10^3 copies/mL.
- •Concurrent use of systemic steroids. Recent or current use of inhaled steroids is not exclusionary.
- •Allergic to immunotherapies and related drugs.
- •Patients with heart disease for which treatment is needed or with poorly controlled hypertension.
- •Hyponatremia: serum sodium level < 125 mmol/L.
- •Baseline serum potassium < 3.5 mmol/L (taking potassium supplements before participating in the study to raise potassium level is acceptable).
- •Previous treatment with chemoradiotherapy, immunotherapy and tumor-targeting drug conducted 2 weeks prior to participation in this study or blood collection.
- •Patients have undertaken immunosuppressor for graft-versus-host disease (GVHD) within 4 weeks before participation in this study or blood collection, or the patient is diagnosed with acute or chronic GVHD.
- •Other severe disease that may restrain patients from participating in this study (e.g. diabetes, severe cardiac dysfunction, myocardial infarction or unstable arrhythmias or unstable angina in recent 6 months, gastric ulcer, active autoimmune disease, etc.).
研究组 & 干预措施
CAR T cell
In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors:
CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction.
Route of administration: Intravenous injection.
Lymphodepletion conditioning:
Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells.
A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.
干预措施: CAR-CD19 T cell (Genetic)
CAR T cell
In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors:
CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction.
Route of administration: Intravenous injection.
Lymphodepletion conditioning:
Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells.
A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.
干预措施: CAR-BCMA T cell (Genetic)
CAR T cell
In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors:
CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction.
Route of administration: Intravenous injection.
Lymphodepletion conditioning:
Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells.
A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.
干预措施: CAR-GPC3 T cell (Genetic)
CAR T cell
In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors:
CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction.
Route of administration: Intravenous injection.
Lymphodepletion conditioning:
Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells.
A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.
干预措施: CAR-CLD18 T cell (Genetic)
CAR T cell
In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors:
CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction.
Route of administration: Intravenous injection.
Lymphodepletion conditioning:
Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells.
A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.
干预措施: Fludarabine (Drug)
CAR T cell
In this study, autologous T cells transduced with a chimeric antigen receptor are used to treat patients with malignant tumors:
CAR-CD19 T cell is for the treatment of B-cell Leukaemia/Lymphoma; CAR-BCMA T cell is for the treatment of Myeloma; CAR-GPC3 T cell is for the treatment of Hepatocellular Carcinoma; CAR-CLD18 T cell is for the treatment of Pancreatic Carcinoma and Adenocarcinoma of Esophagogastric Junction.
Route of administration: Intravenous injection.
Lymphodepletion conditioning:
Lymphodepletion will be conducted several days prior to CAR T cell infusion, which may improve in vivo cell count and survival of T cells.
A combination of fludarabine and cyclophosphamide will be used for lymphodepletion.
干预措施: Cyclophosphamide (Drug)
结局指标
主要结局
Number of participants with CRA T-related adverse events as assessed by CTCAE v4.03
时间窗: 24 weeks
Number of participants with study related adverse events which are defined as laboratory toxicities and clinical events that are possible, likely or definitely related to study treatment at any time from the infusion until week 24, including infusion related toxicity and any toxicity possibly related to CAR T cells.
次要结局
- Engraftment(2 years)
研究者
Kang YU
Head of Department of Hematology
First Affiliated Hospital of Wenzhou Medical University