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Exercise Intolerance Study in Long Chain Fatty Acid Oxidation Disorders

Recruiting
Conditions
Long-chain Fatty Acid Oxidation Disorders
Interventions
Other: Intermittent cardiopulmonary exercise test
Other: Incremental cardiopulmonary exercise test
Other: Echography of upper leg
Other: Hand grip strength with hand dynamometer
Other: Fill in questionnaires regarding physical activity
Registration Number
NCT05924555
Lead Sponsor
Academisch Medisch Centrum - Universiteit van Amsterdam (AMC-UvA)
Brief Summary

In this study, the exercise tolerance in patients with long-chain fatty acid oxidation disorders (LcFAOD) will be compared to that of healthy volunteers who are carefully matched in terms of age, sex, BMI and physical activity score. The study aims to assess exercise capacity using cardiopulmonary exercise (CPX) tests and activity monitoring over a 7-day period. The hypothesis of the study is that LcFAOD patients will exhibit greater oxygen debt during short periods of exercise compared to healthy individuals. These findings will contribute to a better understanding of exercise intolerance in LcFAOD patients and may guide the development of targeted interventions, such as exercise training programs and enable the analysis of effectiveness of (new) treatments.

Detailed Description

Background:Long-chain fatty acid oxidation disorders (LcFAOD) are a group of autosomal recessive disorders that impair the utilization of long-chain fatty acids as an energy source. Exercise intolerance is a commonly reported symptom in adult patients with LcFAOD, but its severity varies significantly among individuals. In addition, little is currently known about the natural progression of exercise intolerance in this group of patients, and reliable outcome measures to assess treatment efficacy remain scarce. In this study, we will evaluate the exercise capacity in LcFAOD patients using intermittent CPX, and determine whether this yields outcome parameters that can be used to determine the course of exercise intolerance over the patients lifetime and evaluate treatment effectiveness.

Hypothesis: Exercise intolerance in LcFAOD patients will be reflected in differences in VO2/VCO2 and heart rate kinetics during short periods of exercise compared to healthy subjects.

Primary Objective: 1. Objectify and quantify exercise intolerance in LcFAOD patients. 2. Determine the relationship between the measured exercise intolerance and patient-reported symptoms, daily life functioning, as well as known markers of disease severity.

Secondary Objective(s): 3. Determine the aetiology of exercise intolerance in LcFAODs. 4. Determine whether the intermittent exercise protocol can be used to establish the course of exercise intolerance and provide clinical outcome measure for future intervention studies.

Methods: Participants will be asked to wear an activity monitor (Activ8) for a period of 7 days. The participants will also perform two cardiopulmonary exercise (CPX) tests (one intermittent and one incremental). During the CPX tests gas exchange, ventilation, blood pressure and cardiac output will be measured and exhaustion level monitored. Blood samples will be taken before the first and after the second CPX test. The grip strength (Maximal Voluntary Contraction) and the leg muscle size (echography) will be assessed. Body composition (using BIA) will be assessed.

Approximately a year later all procedures, including the wearing of the activity monitor, will be repeated in all patients. Some participants will be asked to perform the two exercise tests again within two weeks of their first visit. This way reproducibility of the intermittent exercise protocol can be tested.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
50
Inclusion Criteria
  • LC-FAOD patients:
  • Men and women (age ≥18 years) with a confirmed diagnosis of carnitine palmitoyltransferase 2 (CPT2) deficiency, Carnitine-acylcarnitine translocase (CACT) deficiency, Mitochondrial Trifunctional protein (MTP) deficiency, Very-long-chain acyl-CoA dehydrogenase (VLCAD) deficiency or Long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiency.

Healthy control subjects:

  • Healthy men and women age ≥ 18 years
Exclusion Criteria

LcFAOD patients:

  • Pregnancy
  • Recent acute myocardial infarction (<6 months prior to inclusion)
  • Uncontrolled arrhythmia/severe conduction disorder causing hemodynamic compromise
  • Implantable pacemaker or other cardiac device with complete ventricular pacing
  • Uncontrolled heart failure with hemodynamic compromise
  • Uncontrolled hypertension (Systolic Blood Pressure >150 mmHg and Diastolic Blood Pressure >100 mmHg on repeated measurements)
  • Active infection, anemia, severe renal dysfunction (estimated Glomerular filtration rate < 30 ml/min/1,73m2), or other chronic disease likely to significantly impact exercise performance
  • History of severe asthma or chronic obstructive pulmonary disease
  • Active use of medication likely to affect exercise tolerance

Healthy controls:

  • All abovementioned exclusion criteria for LcFOAD patients
  • History of smoking - History of asthma, chronic obstructive pulmonary disease, heart failure, heart surgery, heart rhythm disorders or congenital heart diseases
  • Chronic illness (including orthopedic, endocrinological, hematological, malignant, gastrointestinal, neurological, muscle or inflammatory disorders) likely to significantly impact on exercise performance
  • > 6 alcohol units per day or > 14 alcohol units per week

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
LcFAOD-patientsIncremental cardiopulmonary exercise testPatients with long-chain Fatty Acid Oxidation including CPT2-, CACT-, VLCAD-, LCHAD-, or MTP-deficiency
LcFAOD-patientsHand grip strength with hand dynamometerPatients with long-chain Fatty Acid Oxidation including CPT2-, CACT-, VLCAD-, LCHAD-, or MTP-deficiency
LcFAOD-patientsEchography of upper legPatients with long-chain Fatty Acid Oxidation including CPT2-, CACT-, VLCAD-, LCHAD-, or MTP-deficiency
Healthy controlsIntermittent cardiopulmonary exercise testControls are matched based on Age, Sex, BMI and comparable activity score assessed using the SQUASH questionnaire
LcFAOD-patientsFill in questionnaires regarding physical activityPatients with long-chain Fatty Acid Oxidation including CPT2-, CACT-, VLCAD-, LCHAD-, or MTP-deficiency
LcFAOD-patientsIntermittent cardiopulmonary exercise testPatients with long-chain Fatty Acid Oxidation including CPT2-, CACT-, VLCAD-, LCHAD-, or MTP-deficiency
Healthy controlsIncremental cardiopulmonary exercise testControls are matched based on Age, Sex, BMI and comparable activity score assessed using the SQUASH questionnaire
Healthy controlsEchography of upper legControls are matched based on Age, Sex, BMI and comparable activity score assessed using the SQUASH questionnaire
Healthy controlsFill in questionnaires regarding physical activityControls are matched based on Age, Sex, BMI and comparable activity score assessed using the SQUASH questionnaire
Healthy controlsHand grip strength with hand dynamometerControls are matched based on Age, Sex, BMI and comparable activity score assessed using the SQUASH questionnaire
Primary Outcome Measures
NameTimeMethod
Ventilation reserve (L)During maximum exercise (max 30 min).

Pulmonary involvement/Skeletal muscle alterations

1. Differences in V'O2/ V'CO2/heart rate kinetics during the intermittent exercise test between LcFAOD patients and healthy control subjectsAt rest (baseline) and during the intermittent CPX test (30 min)
Anaerobic threshold (ml/kg/min)During maximum exercise (max 30 min).

Pulmonary involvement/Cardiac dysfunction/Skeletal muscle alterations

Heart rate reserve (per minute)During maximum exercise (max 30 min).

Cardiac dysfunction

CO2 ventilation equivalent (L/L)During maximum exercise (max 30 min).

Pulmonary involvement/Cardiac dysfunction

Cardiac Output (L/min)During maximum exercise (max 30 min).

Cardiac dysfunction

Muscle size on echography (cm)Baseline

Skeletal muscle alterations

Muscle strength via resistance test (kg)Baseline

Skeletal muscle alterations

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Amsterdam UMC, location AMC

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Amsterdam, Noord-Holland, Netherlands

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