The effect of a one hour bout of games-based activity on adolescent cardiovascular health
Not Applicable
Completed
- Conditions
- Cardio-metabolic healthCirculatory System
- Registration Number
- ISRCTN21855728
- Lead Sponsor
- ottingham Trent University
- Brief Summary
2019 results in https://www.ncbi.nlm.nih.gov/pubmed/30580469 (added 22/11/2019)
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- All
- Target Recruitment
- 39
Inclusion Criteria
1. Male and female participants
2. Aged 10-12 years
3. Healthy and free of non-communicable diseases.
Exclusion Criteria
Non-communicable diseases that may alter rested inflammatory cytokines.
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <br> Inflammatory cytokines are measured acutely using a flow cytometer (Beckman Coulter Gallios™) specific multiplex bead assay (AimPlex™ multiplex assay, YSL Bioprocess Development Company, Pomona, USA) at baseline, immediately, 1 h, 2h and 3 h post-exercise. A final measurement was taken the following morning 24 h post-exercise to assess inflammatory mediators.<br>
- Secondary Outcome Measures
Name Time Method <br> 1. Blood glucose concentration is measured using a commercially available assay (GOD/PAP method, GL364, Randox, Ireland) at baseline, immediately and 1 h post-exercise (30 min following this participants consumed of a standardised lunch), then 30 min, 60 min and 120 min post lunch. A final blood sample was taken the following morning 24 h post-exercise to assess blood glucose.<br> 2. Plasma insulin is measured using a commercially available ELISA (Mercodia Ltd, Sweden) at baseline, immediately and 1 h post-exercise (30 min following this participants consumed of a standardised lunch), then 30 min, 60 min and 120 min post lunch. A final blood sample was taken the following morning 24 h post-exercise to assess plasma insulin.<br>