Molecular Characterization of Blastocystis Isolates From Human by Restriction Fragment Length Polymorphism (RFLP) Analysis

Not yet recruiting

• Conditions: Blastocystis Infections

• Registration Number: NCT05580393
• Lead Sponsor: Sohag University

Brief Summary

Blastocystis is an anaerobic unicellular protozoal parasite infecting the gastrointestinal tract of humans and a wide range of animals. It is one of the most common enteric microorganisms with higher prevalence rates in developing than in developed countries. Feco-oral is the main route of transmission where low socioeconomic conditions, poor hygienic practices, close contact with animals, and drinking contaminated water act as major risk factors. Infection with Blastocystis was demonstrated in both symptomatic and asymptomatic people. For a long period, Blastocystis was considered a commensal organism with no pathogenic role, but recently, many studies linked it to different gastrointestinal symptoms such as nausea, diarrhea, and abdominal pain. Association with irritable bowel syndrome and colorectal cancer was also reported.

This study aims to:

- Identify subtypes of human Blastocystis isolates in Sohag by using RFLP-PCR and provide additional information on the molecular epidemiology of this parasite in our locality.

Detailed Description

Not available

Basic Information
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Recruitment & Eligibility
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Study & Design

Study Timeline

• Status Verified: 2022-10
• Study First Submitted: 2022-10-12
• Study First Submitted QC: 2022-10-13
• Last Update Submitted: 2022-10-13
• Study First Posted: 2022-10-14
• Last Update Posted: 2022-10-14
• Study Start: 2022-11
• Primary Completion: 2023-04
• Study Completion: 2023-11

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Patients from outpatient clinics, Sohag hospitals.

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Exclusion Criteria

• Patients received antiparasitic drugs in the last two weeks

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
The number of cases with subtypes of human Blastocystis isolates in Sohag	14 weeks following the startpoint of the study.	by using RFLP-PCR

Secondary Outcome Measures

Name	Time	Method
The number of cases with Blastocystis infection is recorded.	14 weeks following the startpoint of the study.	By molecular examination of stool samples from 100 patients