MedPath

Ketogenic Diet Health and Longevity

Not Applicable
Completed
Conditions
Ketogenic Dieting
Ketoses, Metabolic
Ketosis
Interventions
Behavioral: Well-formulated Ketogenic Diet
Registration Number
NCT05939011
Lead Sponsor
University of Kansas Medical Center
Brief Summary

The goal of this pilot intervention is to learn about how a well-formulated ketogenic diet (WFKD) impacts various health factors in generally healthy adults. The main questions it aims to answer are:

* Establish whether an 8-week isocaloric, WFKD improves body composition and metabolic biomarkers in adults without chronic disease.

* Examine changes in transcriptomic sequencing pathways pre- and post-WFKD intervention.

* Explore gut microbial changes in adults without chronic disease that consume a WFKD.

Participants will follow a well-formulated ketogenic diet for 8-weeks. Study procedures include:

* Weekly body weight tracking

* Daily urinary ketone assessment

* Pre/post stool samples for gut microbiota analyses

* Pre/post DXA scans

* Diet quality tracking through 3-day food records

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
20
Inclusion Criteria
  • 30 to 55 years
  • Speaks English as primary language
  • BMI 20.0-34.9 kg/m^2
Exclusion Criteria
  • Certain medications (statin, blood pressure, thyroid hormone replacement, etc.)
  • Prior medical diagnosis of type 1 or type 2 diabetes mellitus, cancer requiring chemotherapy or radiation therapy within 5 years, cardiac event, GI disorders, traumatic brain injury/multiple concussions, hypertension, dyslipidemia, or any diagnosis deemed ineligible by study team
  • History of renal stones
  • Currently lactating
  • Already following a ketogenic diet
  • Adherence to a specialized diet regimen that disallows compliance
  • Use of tobacco or tobacco replacement products within 1 year

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Well-formulated ketogenic diet (WFKD)Well-formulated Ketogenic DietThe researchers aim to enroll 20 healthy adults recruited from Kansas City and surrounding areas for an 8-week WFKD diet intervention. Study personnel will obtain baseline (week 0), mid-point (week 4), and end-of-study (8 weeks) blood samples, anthropometrics, and dietary intake data. Fecal microbiome samples and dual energy x-ray absorptiometry (DXA) are collected at baseline and 8-weeks. Blood samples collected at baseline and 8-week visits will be measured for metabolic biomarkers, inflammatory biomarkers, and RNA. The blood sample collected at the 4-week visit will include glucose, insulin, insulin resistance, and beta-hydroxybutyrate to track diet adherence and adjust the diet as needed.
Primary Outcome Measures
NameTimeMethod
Changes in Advanced Lipid PanelBaseline, 8 Week
Changes in Body Composition-- dual x-ray absorptiometry (DXA)Baseline, 8 Week

Measures of tissue distribution

Changes in Waist Circumference (WC)Baseline, 4 Week, 8 Week

Waist circumference (cm)

Changes in Body Mass Index (BMI)Baseline, 4 Week, 8 Week

Ratio of height and weight in kg/m\^2

Changes in Homeostatic model assessment of Insulin Resistance (HOMA-IR)Baseline, 4 Week, 8 Week

Ratio of fasting glucose and fasting insulin with a conversion factor, unitless

Changes in InflammationBaseline, 8 Week

Changes in high sensitivity C-reactive protein (hs-CRP) (mg/L)

Secondary Outcome Measures
NameTimeMethod
Changes in transcriptomic sequencing pathwaysBaseline, 8 weeks

Measured via RNASequencing (RNA-Seq) technique: high-throughput (\~25,000 genes) RNAseq using SE100 Illumina (HiSeq) reads

Gut microbial changesBaseline, 8 weeks

Taxonomic classification of individual operational taxonomic units (OTU) with 97% similarity and produce an OTU table of all identified taxa across all samples

Trial Locations

Locations (1)

University of Kansas Medical Center

🇺🇸

Kansas City, Kansas, United States

© Copyright 2025. All Rights Reserved by MedPath