The Effect of Nutrition on the Microbiome in Pregnant Women and the Use of Micronutrient Supplemented Probiotic Yogurt to Improve Outcomes
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Pregnancy
- Sponsor
- Western University, Canada
- Enrollment
- 77
- Locations
- 1
- Primary Endpoint
- Characterization of gut, oral, vaginal and human milk microbiomes
- Status
- Completed
- Last Updated
- 12 years ago
Overview
Brief Summary
The purpose of this study is to determine whether a difference exists in the gut, oral, vaginal and human milk microbiome of under-nourished pregnant women as compared to healthy and obese pregnant women. In addition, the investigators are setting out to determine if a micronutrient enriched probiotic yogurt can improve the microbiome and pregnancy outcomes of under-nourished pregnant women.
Investigators
Dr. Gregor Reid
Professor
Western University, Canada
Eligibility Criteria
Inclusion Criteria
- •Greater than 12 weeks pregnant
- •between the ages of 18 and 40 years
Exclusion Criteria
- •Less than 12 weeks pregnant
Outcomes
Primary Outcomes
Characterization of gut, oral, vaginal and human milk microbiomes
Time Frame: Monthly for an average of four months
Fecal, oral and vaginal samples were collected on a monthly basis for an average of four months. Human milk samples were collected twice; at birth and one week after. Microbial DNA will be extracted with the MoBio PowerSoil 96-well htp kit. Samples will be sequenced and changes determined by 16s rRNA profiling.
Secondary Outcomes
- Analysis of anthropometric measurements(Monthly on average for four months)
- Assessment of 48-hour Dietary Recalls(Monthly on average for four months)
- Changes in body toxin levels(Before and after intervention (approximately 100 days))
- Changes in blood nutrient levels(before, half-way through and after intervention (approximately 100 days))