Molecular Optimization Via Exercise (MOVE)
- Conditions
- Aging
- Interventions
- Other: Observational
- Registration Number
- NCT06540287
- Lead Sponsor
- Buck Institute for Research on Aging
- Brief Summary
This study is to investigate characteristics of muscle, blood, and urine samples from older adults (ages 65-80) who are either sedentary or athletes performing at an elite level for their age. This observational study will include bio-specimen collection, as well as physical and cognitive function testing and body composition.
- Detailed Description
The study will enroll a total of 80 subjects-20 of each gender for each cohort. Participation in the study involves 3 visits that must take place within 12 weeks. Optional l follow up visits may be completed at 12- and 24- months after completion of the main study.
The first visit is a screening and enrollment visit. At this visit, informed consent will be obtained and a detailed medical history, screening blood sample collected, and physical assessment will be performed (including, but not limited to vital signs, resting ECG, body composition). Additionally, an exercise history interview will be performed to assess for cohort eligibility. Participants will be familiarized with testing equipment during the screening visit and will perform the Astrand-Rhyming submaximal bicycle test. A short physical function testing battery will be performed, including the short physical performance battery, 6 minute walk test, grip strength, 1 rep max leg press, and leg press fatigue testing.
The second visit will include maximal exercise testing, quality of life assessment, and cognitive function testing. Participants will be allocated into their cohort at this visit.
The third visit will include thigh muscle sample collection via needle biopsy and blood sample collection.
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 80
Not provided
Not provided
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Group 2 cohort: Elite Older Athletes (EOA) Observational This cohort will include healthy older adults, aged 65y or over, who are elite athletes for their age (determined by interview and VO2max) Group 1 cohort: Healthy Older Adults (HOA) Observational This cohort will include healthy older adults, aged 65y or over, who participate in less than 60 minutes of structured exercise each week.
- Primary Outcome Measures
Name Time Method Muscle cellular aging - single cell transcriptomics Day 2 Differences between athletes and sedentary in muscle single cell transcriptomic between OEA and HOA groups
Muscle cellular aging - spatial transcriptomics Day 2 Differences between athletes and sedentary in muscle spatial transcriptomic between OEA and HOA groups
- Secondary Outcome Measures
Name Time Method Blood cell immune function Day 2 Differences between athletes and sedentary in blood cell immune function
Physical function - leg press fatigue Day 1 70% of the maximal leg press weight will be used. Subjects will complete as many repetitions as possible of the 70% weight and number of repetitions will be recorded.
Differences between concentration of metabolites in the muscle Day 2 Metabolomic analysis to measure differences in metabolite abundance in EOA vs HOA
Differences between concentration of proteins in the muscle Day 2 Proteomic analysis to measure differences in protein abundance in EOA vs HOA
Differences between function of muscle mitochondria Day 2 Analysis of respiratory function of isolated mitochondria
Blood cell gene expression Day 2 Differences between athletes and sedentary in blood cell gene expression
Physical function - grip strength Day 1 Measured in both hands using a dynamometer
Physical function - short physical performance battery Day 1 SPBB includes balance, gait speed and chair sit tests, consolidated to one score
Physical function - leg strength Day 1 Maximal leg press weight will be determined on a gym machine via progressive increases in weight
Blood metabolites Day 2 Metabolic analysis: differences between athletes and sedentary in blood metabolites
Blood proteins Day 2 Proteomic analysis: differences between athletes and sedentary in blood protein concentration
Physical function - 6 minute walk test Day 1 Measure distance covered in 6 minutes on an indoor walk track
Urine metabolites Day 2 Differences between athletes and sedentary in urine metabolites
Physical function - VO2 max Day 1 Oxygen uptake measured during a ramp test on a bicycle ergometer
Trial Locations
- Locations (1)
Buck Institute for Research on Aging
🇺🇸Novato, California, United States