The Effect of Maximal Exercise on MMP9 and TIMP1 and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms

Not Applicable

Completed

• Conditions: TIMP1; MMP9; MMP9 -1562 C/T; TIMP1 372 T/C
• Interventions: Other: Yo-Yo Intermittent Recovery Test Level 1

• Registration Number: NCT04157556
• Lead Sponsor: Dokuz Eylul University

Brief Summary

Matrix metalloproteinases (MMPs) play a critical role in the remodeling and regeneration of skeletal muscle. Studies which investigate exercise and effects of MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) are contradictory. Also the role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on exercise are unclear. In accordance with this conditions the hypothesis of the study are as follows:

1. Levels of basal serum MMP9 and TIMP1 are different between athletes (volleyball, basketball, handball) and sedentary people.

2. Levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people .

3. Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people.

4. Levels of basal serum MMP9 and TIMP1 are related to MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms.

5. Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise and MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms are different between athletes (volleyball, basketball, handball) and sedentary people.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2019-11-05
• Study First Submitted QC: 2019-11-07
• Study First Posted: 2019-11-08
• Study Start: 2019-11-11
• Primary Completion: 2020-01-03
• Status Verified: 2020-04
• Study Completion: 2020-04-28
• Last Update Submitted: 2020-04-28
• Last Update Posted: 2020-04-30

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 86

Inclusion Criteria

Group of athletes:

• Male basketball players who have been training regularly for at least last 3 months.
• Male volleyball players who have been training regularly for at least last 3 months.
• Male handball players who have been training regularly for at least last 3 months.

Group of sedentary people:

• Male individuals with similar physical characteristics to the group of athletes
• Male individuals who have not exercise regularly for at least last 3 months.

Exclusion Criteria(For two groups):

• Smoking regularly
• Drinking alcohol regularly
• Using drug and antioxidant substance
• Being obese,
• Being sick or having disability

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Group of athletes	Yo-Yo Intermittent Recovery Test Level 1	Age: 18-35 Gender: Male Basketball, volleyball, handball players who have been training regularly for at least last 3 months.
Group of sedentary people	Yo-Yo Intermittent Recovery Test Level 1	Age: 18-35 Gender: Male Individuals with similar physical characteristics to the group of athletes and who have not exercise regularly for at least last 3 months.

Primary Outcome Measures

Name	Time	Method
MMP9 -1562 C/T, TIMP1 372 T/C polymorphisms	Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)	The role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on the effect of acute maximal exercise on serum MMP9 and TIMP1 in athletes and sedentary people.
Level of MMP9 and TIMP1	Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)	The effect of acute maximal exercise on serum MMP9 and TIMP1 levels in athletes and sedentary people.

Secondary Outcome Measures

Name	Time	Method
Level of indicators of muscle damage and inflammation and oxidative stress	Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)	The relationship between primary outcome measures and indicators of muscle damage, inflammation and oxidative stress.; Indicators to be determined: creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Interleukin-6 (IL-6), C- reactive protein (CRP), total antioxidant status (TAS), total oxidant status (TOS), nitric oxide (NO)
Level of blood lipids and lipoproteins, glucose, urea, creatinine, hemogram	Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)	The relationship between primary outcome measures and blood lipids and lipoproteins, glucose, urea, creatinine and hemogram

Trial Locations

Locations (1)

Ege University Faculty of Sports Science; 🇹🇷 İzmir, Turkey