The Effect of Maximal Exercise on Matrix Metalloproteinase Enzyme and Its Inhibitor (MMP9 and TIMP1) and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Dokuz Eylul University
- Enrollment
- 86
- Locations
- 2
- Primary Endpoint
- MMP9 -1562 C/T, TIMP1 372 T/C polymorphisms
Study Overview
Brief Summary
Matrix metalloproteinases (MMPs) play a critical role in the remodeling and regeneration of skeletal muscle. Studies which investigate exercise and effects of MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) are contradictory. Also the role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on exercise are unclear. In accordance with this conditions the hypothesis of the study are as follows:
- Levels of basal serum MMP9 and TIMP1 are different between athletes (volleyball, basketball, handball) and sedentary people.
- Levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people .
- Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people.
- Levels of basal serum MMP9 and TIMP1 are related to MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms.
- Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise and MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms are different between athletes (volleyball, basketball, handball) and sedentary people.
Study Design
- Study Type
- Interventional
- Allocation
- Non Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 35 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Group of athletes:
- •Male basketball players who have been training regularly for at least last 3 months.
- •Male volleyball players who have been training regularly for at least last 3 months.
- •Male handball players who have been training regularly for at least last 3 months.
- •Group of sedentary people:
- •Male individuals with similar physical characteristics to the group of athletes
- •Male individuals who have not exercise regularly for at least last 3 months.
- •Exclusion Criteria(For two groups):
- •Smoking regularly
- •Drinking alcohol regularly
- •Using drug and antioxidant substance
- •Being obese,
- •Being sick or having disability
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
MMP9 -1562 C/T, TIMP1 372 T/C polymorphisms
Time Frame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)
The role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on the effect of acute maximal exercise on serum MMP9 and TIMP1 in athletes and sedentary people.
Level of MMP9 and TIMP1
Time Frame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)
The effect of acute maximal exercise on serum MMP9 and TIMP1 levels in athletes and sedentary people.
Secondary Outcomes
- Level of indicators of muscle damage and inflammation and oxidative stress(Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants))
- Level of blood lipids and lipoproteins, glucose, urea, creatinine, hemogram(Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants))
Investigators
Merve Koca Kosova
research assistant
Dokuz Eylul University