Assessing the Biology of the Injured Lung - Version 1
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 119
- 主要终点
- To identify the mechanisms that occur during lung inflammation, injury and repair and the contribution of immune cells to these processes.
研究概览
简要总结
Respiratory diseases are very common and are the third leading cause of death in England. As such, there is strong interest in understanding how respiratory disease occurs.
This study intends to understand the changes that occur within diseased/injured lungs obtained from humans. The end goal of this will be to create new drugs to help treat these disorders.
Diseased lungs will be obtained from patients receiving a lung transplant. Lungs will either be placed onto a heart-lung machine, or surgically cut in order to create a model of the lung that can be used experimentally in the laboratory. Using a heart-lung machine, lungs can be maintained outside of the human body for a maximum of 12 hours, allowing the direct assessment of the organ. Using this procedure, we aim to understand the processes that occur within a disease, as well as during repair. Using the model of the lung, we will look at how the body's immune system interacts within a diseased lung.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients must be able to provide informed consent
- •Patients must be aged between 18-80 years of age
排除标准
- •Patients under 16 or over 70 years of age
结局指标
主要结局
To identify the mechanisms that occur during lung inflammation, injury and repair and the contribution of immune cells to these processes.
时间窗: Prior to lung transplantation.
Cytokines and chemokines within lungs will be assessed to determine inflammatory profiles. This will be done using luminex arrays that allow quantification of a range of immunologic molecules in bronchoalveolar lavage and perfusate samples. This will provide a broad overview of the prevailing local tissue environment. We will also assess this following immunomodulation as this may be significantly altered by the presence of immunomodulatory therapeutics.
次要结局
- Cut sections of the lung to culture in an in vitro lung model to assess the effect of various different mechanisms of injury on the function and characteristics of immune cells.(Prior to lung transplantation)
- Characterise the migration and functional profile of immune cells within the inflamed/damaged lung.(Prior to lung transplantation)
- Identify new therapeutic targets/agents that can reduce the incidence/severity of inflammatory respiratory conditions.(Prior to lung transplantation)
- Assess the effectiveness of therapeutic agents that can repair lung damage.(Prior to lung transplantation)
- Trial therapeutic agents that can modulate the effect of immune cells in response to lung tissue injury.(Prior to lung transplantation)