Potential of patient derived vascular cells for usage in vascular tissue engineering

Completed

Conditions: blood vessel disease
vascular disease
10011082
10003216

Registration Number: NL-OMON38188

Lead Sponsor: niversiteit Maastricht

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Completed

Sex: Not specified

Target Recruitment: 95

Inclusion Criteria

All groups: Age>18 years
Group A: Patients undergoing elective bypass surgery for coronary arterial disease in which the saphenous vein is used as bypass graft.
Group B: Patients receiving an AV-shunt in which a native arm vein is used for creation of the loop and which are having renal failure. Patients undergoing kidney transplantation.
Group C: Age/sex-matched patients undergoing surgical procedures in which small vessels are usually ligated or removed. Examples of these surgical procedures include thyroidectomy, inguinal hernia repair, or sarcoma resection.

Exclusion Criteria

All groups: Age <18 years
Patients who will not provide informed consent.
Group C: History of cardiovascular disease, e.g. hypertension, hypercholesterolemia, diabetes, angina pectoris, myocardial infarction, cerebrovascular accident or peripheral arterial disease and chronic kidney disease. Smoking, or history of smoking in the last 15 years.

Study & Design

Study Type: Observational invasive

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>During in vitro cell culturing, basic characteristics will be determined and<br /><br>functional tests in high-throughput assays will be performed. The main endpoint<br /><br>will be reproductive capacity of each badge of cells.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>Secondary parameters will be cellular adhesion and thrombogenicity for EC and<br /><br>extracellular matrix production for SMC. Besides the cellular in vitro assays,<br /><br>biomarkers for endothelial dysfunction will be measured in plasma. We aim to<br /><br>measure sICAM, sVCAM, vWF and thrombomodulin. Based on the outcomes of above<br /><br>mentioned assays, interindidivual variability will be determined by calculating<br /><br>the coefficient of variation.</p><br>