The role of the airway smooth muscle layer in asthma
Completed
- Conditions
- allergic asthma, airway smooth muscle, genomics, microarray, gene expression, Forced Oscillation Techniqueallergisch astma, luchtweg glad spierweefsel, gen expressie
- Registration Number
- NL-OMON24761
- Lead Sponsor
- Academic Medical Center AmsterdamContactperson:P.J. SterkDept. of Pulmonology (F5-259)Academic Medical CenterUniversity Hospital of Amsterdam Meibergdreef 9NL-1105AZ, Amsterdam, The NetherlandsTel: 31-20-566 4356Fax: 31-20-566 9001Email: p.j.sterk@amc.uva.nl
- Brief Summary
/A
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 48
Inclusion Criteria
Asthmatic patients:
1. Age between 18 – 50 years
Exclusion Criteria
Both groups of patients:
1. History of clinical significant hypotensive episodes or symptoms of fainting, dizziness, or light-headedness
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method A genomic analysis of the airway smooth muscle layer of asthmatics and healthy, non-asthmatics by using laser capture microdissection (LCM) technique.
- Secondary Outcome Measures
Name Time Method The profiles of ASM will be associated with physiologic parameters obtained by lung function tests, eNO, and FOT.<br /><br>The profiles of ASM will be associated with biochemical parameters obtained by immunohistochemistry.<br /><br>The presence and type of bronchial pathogens will be associated with clinical severity, and with the degree and profile of airways inflammation, airway remodelling and airway hyperresponsiveness by viral real-time multiplex PCR and bacterial PCR analysis.<br>