Evaluation of the Relationship Between Bruxism and Tryptophan Metabolites

Not Applicable

Completed

• Conditions: Tryptophan Metabolism Alterations; Bruxism; Anxiety State
• Interventions: Diagnostic Test: Metobolites serum level measurement; Behavioral: Anxiety and stress level measurement

• Registration Number: NCT05760482
• Lead Sponsor: Selcuk University

Brief Summary

Psychological causes are often cited as the most important of the underlying factors for bruxism. However, there are very few studies that can objectively demonstrate this. There are studies that are generally based on subjective data, that is, on questionnaires that indicate anxiety or stress. Recent studies have shown that tryptophan and its metabolites are associated with psychological health. In this study, researchers will measure the serum values of metabolites in the tryptophan pathway. Also, researchers will observe whether these metabolite levels differ significantly in patients with and without bruxism.

Detailed Description

Bruxism is a common health problem that occurs throughout life and has a prevalence of 9% to 31% among adults. This common muscle disorder, characterized by teeth grinding or clamping, which can occur during sleep or day, is considered multifactorial with potential effects on the central nervous system. A possible relationship between mental health and bruxism has been investigated due to the indication of psychological factors such as anxiety and stress at the onset of bruxism. It has been reported that when the presence of mental disorders causes changes in the regulation of the central nervous system, they can act as a trigger point for bruxism.

Depression and anxiety disorders are the most common mental disorders studied and are expected to be the most common chronic condition by 2050. The emerging field of nutritional psychiatry offers some opportunities for clinical intervention for individuals suffering from depression and anxiety. L-tryptophan, a precursor of serotonin, is expected to contribute to the correction of depressive and anxious moods.

The primary catabolic pathway of tryptophan is the pathway of kynurenine, which is responsible for about 95-99% of tryptophan metabolism in the body. Tryptophan turns into kynurenine in the kynurenine pathway and is divided into two pathways. The first forms kynurenic acid, while the other forms 3-hydroxykynurenine (3-HK), 3-hydroxyanthranylic acid (3-HAA), and quinolinic acid (QA), respectively. The first pathway is considered neuroprotective, while the second pathway is known as neurotoxic.

In studies trying to explain the link between tryptophan and its metabolites and depression, they have emphasized that depression is caused by an imbalance in the kynurenine pathway and that the amount of quinolinic acid, which is neurotoxic, increases, while peripheral kynurenine, which is neuroprotective, decreases. It has been shown that the neurotoxic metabolites formed in the kynurenine itself and its pathway may be associated with depression and anxiety. There are researchers who think that changes in these parameters and their proportions to each other indicate different psychological states.

Changes in serum levels of these metabolites in cases of stress and anxiety have been supported by the studies mentioned above. Although most authorities assume that bruxism is based on psychological factors, the studies supporting them are usually anxiety and stress-focused survey studies based on subjective data. This assumption; researchers could not find a study comparing it with objective data. This study aims to present a possible relationship between bruxism and tryptophan metabolites in volunteer individuals with and without bruxism. The researchers' initial hypothesis is that "Changes in tryptophan and catabolites play a role in the etiology of bruxism."

Study Timeline

• Study First Submitted: 2023-02-26
• Study First Submitted QC: 2023-02-26
• Study First Posted: 2023-03-08
• Study Start: 2023-09-04
• Primary Completion: 2024-05-31
• Status Verified: 2024-06
• Study Completion: 2024-06-01
• Last Update Submitted: 2024-06-05
• Last Update Posted: 2024-06-06

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 92

Inclusion Criteria

• age range 18-50
• applying to our clinic with the complaint of bruxism
• no psychological drug
• no active orthodontic treatment
• no musculoskeletal disease
• no removable prosthesis
• no obstructive sleep apnea syndrome
• "Class I" according to RDC/TMD classification
• "probable" according to bruxism classifiacation
• accept to fill out the questionnaire
• accept to give a blood sample
• being volunteer
• accept to sign the informed consent

Exclusion Criteria

• indicate to quit the study at anytime

• not fully fill out the questionnaire

• encountering any complication during taking a blood sample

• can not take an adequate blood sample

• presenting any below diseases;

  1. acute myocardial infarction
  2. pulmonary emboli
  3. deep venous thrombosis
  4. heart failure
  5. sepsis
  6. cerebral edema
  7. hypertensive encephalopathy
  8. hypoglycemia
  9. hepatic encephalopathy
  10. fluid-electrolyte disturbances
  11. acute-chronic renal failure

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Bruxism Group	Anxiety and stress level measurement	-
Control Group	Metobolites serum level measurement	-
Control Group	Anxiety and stress level measurement	-
Bruxism Group	Metobolites serum level measurement	-

Primary Outcome Measures

Name	Time	Method
serum kynurenic acid level measurement	blood sample will be taken 5 minutes after the patient sits the dental unit	serum kynurenic acid level measurement
serum quinolinic acid level measurement	blood sample will be taken 5 minutes after the patient sits the dental unit	serum quinolinic acid level measurement
serum kynurenin level measurement	blood sample will be taken 5 minutes after the patient sits the dental unit	serum kynurenin level measurement
serum tryptophane level measurement	blood sample will be taken 5 minutes after the patient sits the dental unit	serum tryptophane level measurement
serum 3-hydroxyanthranilic level measurement	blood sample will be taken 5 minutes after the patient sits the dental unit	serum 3-hydroxyanthranilic(3-HAA) level measurement
serum 3-hydroxykynurenin level measurement	blood sample will be taken 5 minutes after the patient sits the dental unit	serum 3-hydroxykynurenin(3-HK) level measurement

Trial Locations

Locations (1)

Selcuk University, Faculty of Dentistry; 🇹🇷 Konya, Selçuklu, Turkey