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Effect of Probiotics on Infantile Colic Symptoms

Phase 2
Recruiting
Conditions
Infantile Colic
Interventions
Dietary Supplement: Probiotic
Dietary Supplement: Placebo
Registration Number
NCT06385054
Lead Sponsor
Lallemand Health Solutions
Brief Summary

The aim of this clinical trial is to assess the impact of B. lactis B94 on participants with infantile colic. It is hypothesized that participants given the probiotic formula will have a significant reduction in their crying duration compared to participants receiving the placebo, after 4 weeks of intervention.

Detailed Description

The goal of this clinical trial is to evaluate the potential beneficial effects of B. lactis B94 on the symptoms of infantile colic.

Participants diagnosed with infantile colic will be recruited to participate in this randomized, double-blind, placebo controlled, two-armed parallel study. Eligible participants will be enrolled in this study for 6 weeks, and will undergo a 1-week run-in baseline period followed by a 4-week interventional period, then a 1-week follow-up period.

The study will consist of 3 in-person visits and 4 phone calls.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
70
Inclusion Criteria

I1. Healthy male or female. I2. Age ≤ 8 weeks old. I3. Diagnosis of infantile colic according to the Rome IV criteria. Infants must show cry or fuss behavior for 3 or more hours per day, during 3 or more days in 7 days.

I4. Exclusively breastfeeding and planning to breastfeed for the duration of the study.

I5. With a written informed consent signed by the father and mother or legal guardian.

I6. With parents willing to complete questionnaires, records, and diaries associated to the study and to complete all clinical visits and scheduled telephone calls.

I7. At least one of the legal representatives is affiliated with a social security scheme.

Exclusion Criteria

E1. Birthweight < 2500 g. E2. Gestational age < 37 weeks. E3. Apgar score at 5 minutes < 7. E4. Partially or fully formula fed infants except for the 4 first days after birth.

E5. Stunted growth/weight loss (< 100 g/week from birth to last reported). E6. Gastroesophageal reflux disease, short bowel syndrome, chronic intestinal diseases, or gastrointestinal malformations.

E7. Diagnosed or reported fever and/or infectious diseases, or current systemic infections, or history of congenital infections.

E8. Genetic diseases and chromosomal abnormalities. E9. Metabolic diseases or pancreatic insufficiency. E10. Immunodeficiency. E11. Neurological diseases. E12. Suspected or confirmed food allergies and intolerances. E13. Mother who consumed antacid medications (proton pump inhibitor and H2 receptor antagonist) within the month before birth and intend to consume any during the trial.

E14. Use of probiotics, prebiotics, antibiotics, or gastric acid inhibitors by the infant at any time from birth to the moment of screening and during the trial.

E15. Use of anti-colic medication at any time from birth to the moment of screening.

E16. Use of probiotics and prebiotics supplements by the mother between the birth of their infant and the moment of screening and intend to consume any during the trial.

E17. Currently enrolled in another clinical study or having participated in another clinical from birth to the moment of screening.

E18. Whose legal representatives have psychological or linguistic incapability to sign the informed consent.

E19. Impossibility to contact the legal representatives in case of emergency.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
ProbioticProbioticParticipants in this group will be randomized to receive the probiotic B. lactis B94 for 4 weeks.
PlaceboPlaceboParticipants in this group will be randomized to receive a placebo for 4 weeks.
Primary Outcome Measures
NameTimeMethod
Change in daily crying duration4 weeks

Proportion of infants who experience a decrease of mean daily crying duration of at least 50% after 4 weeks of intervention, in placebo versus probiotic groups.

Secondary Outcome Measures
NameTimeMethod
Change in crying patterns4 weeks

Proportion of infants who experience a decrease of mean daily crying duration of at least 50% or at least 25%, mean daily number of crying episodes and variation in mean daily crying duration after 1, 2, 3, 4 weeks of intervention, in placebo versus probiotic groups.

Change in sleep duration4 weeks

Variation in the mean daily duration of the infant's sleep after 1, 2, 3, 4 weeks of intervention in probiotic versus placebo groups.

Change in gut microbiota composition4 weeks

Gut microbiota composition (α-diversity, β-diversity, and relative abundance at the genus level) and variation in gut microbiota composition (α-diversity, β-diversity, and relative abundance at the genus level) after 4 weeks of intervention in probiotic versus placebo groups (via 16S sequencing).

Probiotic strain recovery4 weeks

Recovery of the probiotic strain in infant stool samples (qPCR) after 4 weeks of intervention.

Change in maternal quality of life4 weeks

Variation in the maternal score of the mother quality-of-life questionnaire (SF- 36) after 4 weeks of intervention in probiotic versus placebo groups.

Change in bowel habits4 weeks

General perception of colic evolution (total recovery / better / worse / no change), infant bowel movement frequency (mean daily number of stools) and stool consistency (rate (%), Amsterdam infant stool scale) after 1, 2, 3, 4 weeks of intervention in probiotic versus placebo groups.

Trial Locations

Locations (9)

Centre Hospitalier de Boulogne-sur-Mer

🇫🇷

Boulogne-sur-Mer, France

CHU Caen Normandie

🇫🇷

Caen, France

Cabinet privé Dr Regimbart-Trubuil Christine

🇫🇷

Bécon-les-Granits, France

CHU Grenoble Alpes

🇫🇷

La Tronche, France

Hôpital Saint Vincent de Paul

🇫🇷

Lille, France

Centre Hospitalier de Montauban

🇫🇷

Montauban, France

CHU de NANTES

🇫🇷

Nantes, France

Biofortis, Unité d'investigation Clinique

🇫🇷

Saint-Herblain, France

Centre Hospitalier du val d'Ariège

🇫🇷

Saint-Jean-de-Verges, France

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