Embryo Development in Microfluidics System of Culture

Phase 2

• Conditions: Infertility
• Interventions: Device: Static culture; Device: SMART dynamic system of embryo culture

• Registration Number: NCT01003548
• Lead Sponsor: Federal University of São Paulo

Brief Summary

Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.

Detailed Description

Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.

Study Timeline

• Status Verified: 2009-10
• Study Start: 2009-10
• Study First Submitted: 2009-10-27
• Study First Submitted QC: 2009-10-27
• Last Update Submitted: 2009-10-27
• Study First Posted: 2009-10-28
• Last Update Posted: 2009-10-28
• Primary Completion: 2010-10
• Study Completion: 2011-10

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Female
• Target Recruitment: 400

Inclusion Criteria

• Age between 21 and 35 years old
• No more than 1 previous IVF failure
• Absence of physical conditions that can interfere in oocyte quality
• At least 8 zigotes 24 hours after fertilization

Exclusion Criteria

• Use of epididimary or testicular sperm
• Sperm or egg donation
• Untreated systemic diseases

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Static culture	Static culture	Embryos individually cultured in microdrops of 40 microliters of G-IVFplus series V
Smart plataform	SMART dynamic system of embryo culture	Embryos culture in dynamics microfluidic culture system

Primary Outcome Measures

Name	Time	Method
Embryo morphology on days 2 and 3 of embryo culture according to the criteria described by Lucinda Veeck	Embryo culture for 3 days before transference to the uterus

Secondary Outcome Measures

Name	Time	Method
Implantation rate and pregnancy rate	24 month

Trial Locations

Locations (1)

Departamento de Ginecologia; 🇧🇷 Sao Paulo, Brazil