Exercise as a countermeasure against the effects of ageing on muscle mitochondria, diffusive oxygen transport and muscle volume
- Conditions
- Healthy ageing
- Registration Number
- NL-OMON56510
- Lead Sponsor
- Vrije Universiteit
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 60
In order to be eligible to participate in this study, young sedentary
participants must meet all of the following criteria:
• Aged between 18-30 years
• Male or female
• Not currently engaging in any formal exercise training or competitive sports
• No chronic health conditions likely to affect exercise tolerance or the
physiological responses to exercise
In order to be eligible to participate in this study, young trained
participants must meet all of the following criteria:
• Aged between 18-30 years
• Male or female
• Currently engaging in formal training (at least 3 times per week) in
competitive endurance sports
• No chronic health conditions likely to affect exercise tolerance or the
physiological responses to exercise
In order to be eligible to participate in this study, older sedentary
participants must meet all of the following criteria:
• Aged between 50-65 years
• Male or female
• Not currently engaging in any formal exercise training or competitive sports
• No chronic health conditions likely to affect exercise tolerance or the
physiological responses to exercise
In order to be eligible to participate in this study, older trained
participants must meet all of the following criteria:
• Aged between 50-65 years
• Male or female
• Currently engaging in formal training (at least 3 times per week) in
competitive endurance sports
• No chronic health conditions likely to affect exercise tolerance or the
physiological responses to exercise
• Age that falls outside of 18-30 years (young groups) or 50-65 years
(middle-aged groups)
• Inability to provide informed consent
• History of claustrophobia
• Ineligibility to perform the exercise test described in this study protocol
or follow instructions
• Contraindication for MRI (e.g. pacemaker, claustrophobia; see F1
vragenlijsten screening MRI Amsterdam )
• Being under investigation for non-diagnosed disease at the time of
investigation
• Risk factors for exercise testing registered by a Dutch version of the
pre-participation questionnaire (American college of sports medicine and
American Heart association). Possible risk factors will be discussed with a
medical specialist or general practitioner before a subject can be included
• BMI >30 due to adiposity, since this is known to cause difficulties in
obtaining muscle biopsies and NIRS measurements
• Vastus lateralis adipose tissue thickness >10 mm due to difficulties in
obtaining high quality NIRS data
• Pregnancy
• Are current smokers or have been a regular smoker within the last 12 months
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>The primary outcomes are: maximal oxygen uptake (V*O2max), muscle volume,<br /><br>muscle strength, muscle power, muscle diffusing capacity for oxygen (DmO2), and<br /><br>indicators of mitochondrial morphology.</p><br>
- Secondary Outcome Measures
Name Time Method <p>The secondary parameters can be categorised into the following groups of<br /><br>variables: exercise capacity, mitochondrial content and morphology, ex-vivo<br /><br>mitochondrial respiratory function, in vivo mitochondrial energetics, histology<br /><br>and immunohistochemistry, muscle morphological variables, mitochondrial<br /><br>dynamics proteins, and physical activity status. Further details are given in<br /><br>the protocol section 8.1.2.</p><br>