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Antibiotic and Probiotic Therapies in the Adjuvant Treatment of the Chronic Periodontitis.

Not Applicable
Completed
Conditions
Chronic Periodontitis Complex
Interventions
Registration Number
NCT03692819
Lead Sponsor
Universidade Estadual Paulista Júlio de Mesquita Filho
Brief Summary

Currently, with the increase of bacterial resistance to antibiotics associated with its side effects, it is necessary to find other adjuncts to combat the disease that are an alternative to the use of antibiotics. The use of probiotics has been proposed in the literature as an adjunct therapy for the treatment of periodontal diseases, however, few controlled and randomized clinical studies have been performed. The objective of this double-blind randomized controlled trial will be to evaluate the response of clinical and microbiological periodontal parameters to the comparison of two different adjuvant therapies (antibiotic and probiotic) for the treatment of stages II and III grade B periodontitis.

Detailed Description

Currently, with the increase of bacterial resistance to antibiotics associated with its side effects, it is necessary to find other adjuncts to combat the disease that are an alternative to the use of antibiotics. The use of probiotics has been proposed in the literature as an adjunct therapy for the treatment of periodontal diseases, however, few controlled and randomized clinical studies have been performed. The objective of this double-blind randomized controlled trial will be to evaluate the response of clinical and immunological periodontal parameters to the comparison of two different adjuvant therapies (antibiotic and probiotic) for the treatment of stages II and III grade B periodontitis. Patients will be randomized into 3 groups: Group 1 (n=15): periodontal debridement, Group 2 (n=15): periodontal debridement associated with antibiotic (Metronidazole 400 mg + Amoxicillin 500mg) and Group 3 (n=15): periodontal debridement associated with probiotic (Lactobacillus reuteri). Clinical (plaque index, gingival index, probing depth, gingival recession, relative clinical attachment level and periodontal inflamed surface area (PISA) index) and immunological data obtained before and after periodontal therapy (baseline, 30 and 90) will be consolidated and made available on average ± standard deviation and normality tested using the Shapiro-Wilk test. For statistical analysis, the software BioEstat 5.0 (Belém, PA, Brazil) will be used, with p-value \< 0,05.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
45
Inclusion Criteria
  • Individuals between 35 and 50 years of age;
  • Be diagnosed with generalized chronic periodontitis: present a loss of interproximal clinical insertion> 3 mm in 2 nonadjacent teeth and loss of interproximal clinical insertion ≥5 mm, in 30% or more of the teeth present;
  • Present at least 16 teeth;
  • Present good systemic health;
  • Agree to participate in the study and sign the informed consent form
Exclusion Criteria
  • Patients with systemic problems (cardiovascular changes, blood dyscrasias, immunodeficiency, among others), which do not indicate the periodontal procedure;
  • Have undergone periodontal treatment in the last twelve months;
  • Have used antibiotics and/or anti-inflammatories for the past six months;
  • Smoking patients;
  • Pregnancy or Lactation;
  • Chronic use of medications that may alter the response of periodontal tissues.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Metronidazole and AmoxicillinAmoxicillin 500 MgPeriodontal debridement treatment in a single session Metronidazole 400mg + Amoxicillin 500mg every 8 hours for 7 days.
placeboPlacebo Oral TabletPeriodontal debridement treatment will be performed in a single session after the therapy will be administered the Placebo Oral Tablet twice a day for 21 days.
Lactobacillus reuteriLactobacillus reuteri Oral DropsPeriodontal debridement treatment in a single session Lactobacillus reuteri Oral Drops twice a day for 21 days.
Primary Outcome Measures
NameTimeMethod
Probing Depth changeBaseline, 30 and 90 days

The change in the probing depth will be measure in millimeters before and after the treatment.

Secondary Outcome Measures
NameTimeMethod
Gingival RecessionBaseline, 30 and 90 days

Evaluated in milimeters before and after the treatment.

Clinical Attachment LevelBaseline, 30 and 90 days

Evaluated in milimeters before and after the treatment.

Periodontal Inflamed Surface Area (PISA)Baseline, 30 and 90 days

Is calculated from data collected on probing the periodontal pocket and are placed in a table which gives the value of the periodontal inflamed surface area.

Microbiological ParametersBaseline, 30 and 90 days

crevicular fluid samples will be analyzed by PCR (real time PCR) for detection and quantification of: Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Lactobacillus reuteri.

Gingival IndexBaseline, 30 and 90 days

Number of teeth affected before and after the treatment.

Plaque IndexBaseline, 30 and 90 days

Number of teeth affected before and after periodontal treatment.

Trial Locations

Locations (1)

Maria Jardini

🇧🇷

São José dos Campos, São Paulo, Brazil

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