Atrificial Shrinkage of Blastocyst Before In Vitro Fertilization

Not Applicable

Completed

• Conditions: Infertility
• Interventions: Device: ICSI with laser pulse; Device: ICSI with micro needle technique

• Registration Number: NCT04125017
• Lead Sponsor: Aljazeera Hospital

Brief Summary

Vitrification is a modern cryopreservation technique based on sudden cooling of reproductive cells to a temperature of -196 ° C in seconds that produces glass-like solidification of a solution without crystallization, but by extreme elevation in viscosity during freezing

Detailed Description

Vitrification is a modern cryopreservation technique based on sudden cooling of reproductive cells to a temperature of -196 ° C in seconds that produces glass-like solidification of a solution without crystallization, but by extreme elevation in viscosity during freezing Unlike the slow freezing method, vitrification has better results in a complete elimination of ice crystal inside the cells as well as in the surrounding solution -Also, it has a significantly higher post-thawing survival rate in comparison to slow freezing method

Study Timeline

• Study Start: 2019-01-01
• Study First Submitted: 2019-10-10
• Study First Submitted QC: 2019-10-10
• Study First Posted: 2019-10-14
• Primary Completion: 2020-01-04
• Study Completion: 2020-02-05
• Status Verified: 2023-10
• Last Update Submitted: 2023-10-06
• Last Update Posted: 2023-10-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 409

Inclusion Criteria

• women with infertility from 20 to 44 years

Exclusion Criteria

• women with age more than 44 or less than 20

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Micro-needle Technique	ICSI with laser pulse	effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
effect of using LASER pulse	ICSI with laser pulse	effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
effect of using LASER pulse	ICSI with micro needle technique	effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
Micro-needle Technique	ICSI with micro needle technique	effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability

Primary Outcome Measures

Name	Time	Method
The number of women who will have positive pregnancy test	2 months	The number of women who will come pregnant after each technique

Trial Locations

Locations (1)

Adam; 🇪🇬 Giza, Egypt