Comparative Study Between Two Techniques for Artificial Shrinkage of Blastocysts Prior to Vitrification: LASER Pulse Versus Micro-needle Technique in Increasing Chemical, Clinical Pregnancy and Live Birth Rates After ICSI
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Aljazeera Hospital
- Enrollment
- 409
- Locations
- 1
- Primary Endpoint
- The number of women who will have positive pregnancy test
Study Overview
Brief Summary
Vitrification is a modern cryopreservation technique based on sudden cooling of reproductive cells to a temperature of -196 ° C in seconds that produces glass-like solidification of a solution without crystallization, but by extreme elevation in viscosity during freezing
Detailed Description
Vitrification is a modern cryopreservation technique based on sudden cooling of reproductive cells to a temperature of -196 ° C in seconds that produces glass-like solidification of a solution without crystallization, but by extreme elevation in viscosity during freezing Unlike the slow freezing method, vitrification has better results in a complete elimination of ice crystal inside the cells as well as in the surrounding solution -Also, it has a significantly higher post-thawing survival rate in comparison to slow freezing method
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Supportive Care
- Masking
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
Eligibility Criteria
- Ages
- 20 Years to 44 Years (Adult)
- Sex
- Female
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •women with infertility from 20 to 44 years
Exclusion Criteria
- •women with age more than 44 or less than 20
Arms & Interventions
effect of using LASER pulse
effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
Intervention: ICSI with laser pulse (Device)
effect of using LASER pulse
effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
Intervention: ICSI with micro needle technique (Device)
Micro-needle Technique
effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
Intervention: ICSI with laser pulse (Device)
Micro-needle Technique
effect of using LASER pulse versus micro-needle use in the artificial shrinkage of blastocysts as a previous step before vitrification in regarding their effect on embryo viability
Intervention: ICSI with micro needle technique (Device)
Outcomes
Primary Outcomes
The number of women who will have positive pregnancy test
Time Frame: 2 months
The number of women who will come pregnant after each technique
Secondary Outcomes
No secondary outcomes reported
Investigators
Mahmoud Alalfy
Consultant of Obstetrics and Gynecology
Aljazeera Hospital