The Bacterial Secretome in Crohn's Disease and Ulcerative Colitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 74
- 试验地点
- 1
- 主要终点
- Concentration of proinflammatory cytokines of bacterial fractions in IBD compared to healthy controls
研究概览
简要总结
Secreted bacterial effectors produced by the intestinal microbiota are in part responsible for the proinflammatory effect of the fecal content in inflammatory bowel disease (IBD) patients.
详细描述
Fecal colonic content will be collected by suction during routine colonoscopy. From the fecal content different fractions (containing soluble bacterial fractions and membrane bound bacterial fractions) will be processed. These bacterial fractions (also termed as bacterial secretome) will be tested for proinflammatory potential in tissue culture assays including epithelial colonic cell lines and dendritic/monocytic cell lines. The main outcome parameter will be the secretion of proinflammatory cytokines from the tissue culture.
For this purpose the investigators will test different groups of patients including patients with active/inactive Crohn's disease or ulcerative colitis, patients with other inflammatory conditions of the colon, patients with colorectal carcinoma and healthy controls undergoing colonoscopy.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Concentration of proinflammatory cytokines of bacterial fractions in IBD compared to healthy controls
时间窗: One day
Measurement of proinflammatory cytokines (f.e.: IL-8) in epithelial and dendritic tissue culture assays
次要结局
- Concentration of proinflammatory cytokines of bacterial fractions in relation to disease type(one day)
- Concentration of proinflammatory cytokines of bacterial fractions in relation to disease activity(One day)
- Concentration of proinflammatory cytokines of bacterial fractions in other colonic diseases(One day)