Regulation of Postprandial Nitric Oxide Bioavailability and Vascular Function By Dairy Milk
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 23
- 试验地点
- 2
- 主要终点
- Vascular Endothelial Function
研究概览
简要总结
Cardiovascular disease (CVD) is the leading cause of death in the United States. Short-term increases in blood sugar, or postprandial hyperglycemia (PPH), affect blood vessel function and increase the risk of CVD. Greater intakes of dairy foods have been associated with a lower risk of CVD, but whether these effects occur directly or indirectly by displacing foods in the diet that might increase CVD risk is unclear. The health benefits of dairy on heart health are at least partly attributed to its ability to limit PPH and resulting PPH-mediated responses leading to vascular dysfunction. This provides rationale to further investigate dairy as a dietary strategy to reduce PPH and risk for CVD. The objective of this study is to define the extent to which dairy milk, and its whey and casein protein fractions, protect against postprandial vascular dysfunction by reducing oxidative stress responses that limit nitric oxide bioavailability to the vascular endothelium in adults with prediabetes.
详细描述
This study consists of four, 3-hour postprandial trials in response to consuming the following dietary treatments: 1. oral glucose challenge, 2. oral glucose challenge in combination with non-fat milk, 3. oral glucose challenge in combination with whey protein isolate, and 4. oral glucose challenge in combination with sodium caseinate. For three days preceding each trial, participants will be provided all meals to standardize physiologic responses to test meals. On each trial day, vascular function will be assessed and blood samples collected prior to and at 30 minute intervals for 3 hours following test meal ingestion.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •hemoglobin A1c 5.7-6.4%
- •non-dietary supplement user
- •no medications affecting vasodilation, inflammation, or energy metabolism
- •individuals having blood pressure <140/90 mmHg and total cholesterol <240 mg/dL
排除标准
- •unstable weight (±2 kg)
- •vegetarian or dairy allergy
- •alcohol intake >3 drinks/day or >10 drinks/week
- •≥7 hours/week of aerobic activity
研究组 & 干预措施
Glucose with Non-fat Milk
This study day will last approximately three hours and will be separated from the other arms by four days for men and one month for women.
干预措施: Glucose with Non-fat Milk (Other)
Glucose with Whey Protein Isolate
This study day will last approximately three hours and will be separated from the other arms by four days for men and one month for women.
干预措施: Glucose with Whey Protein Isolate (Other)
Glucose with Sodium Caseinate
This study day will last approximately three hours and will be separated from the other arms by four days for men and one month for women.
干预措施: Glucose with Sodium Caseinate (Other)
Glucose
This study day will last approximately three hours and will be separated from the other arms by four days for men and one month for women.
干预措施: Glucose (Other)
结局指标
主要结局
Vascular Endothelial Function
时间窗: Area under curve for FMD for three hours (0, 30, 60, 90, 120, 180 minutes)
Flow mediated dilation (FMD) of the brachial artery, calculated as FMD AUC for 0-180 minutes (change from baseline)
次要结局
- Cholecystokinin (CCK)(CCK area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- Nitrite/Nitrate (NOx)(Area under curve for nitrite/nitrate for three hours (0, 30, 60, 90, 120, 180 min) (change from baseline))
- Plasma Glucose(Area under the curve for glucose for three hours (0, 30, 60, 90, 120, 180 minutes) (change from baseline))
- Arginine (ARG)(ARG area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- Asymmetric Dimethylarginine/Arginine (ADMA/ARG)(ADMA/ARG area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- Symmetric Dimethylarginine/Arginine (SDMA/ARG)(SDMA/ARG area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- Tetrahydrobiopterin/Dihydrobiopterin (BH4/BH2)(Plasma BH4/BH2 concentration area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- 8-isoprostaglandin-F2a(8-isoprostaglandin-F2a area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- 8-isoprostaglandin-F2a/Arachidonic Acid(8-isoprostaglandin-F2a/Arachidonic acid area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- Arachidonic Acid(Arachidonic acid area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
- Malondialdehyde (MDA)(Area under curve for MDA for three hours (0, 30, 60, 90, 120, 150, 180 min.) (change from baseline))
- Insulin(Insulin area under the curve for 3 hours (0, 30, 60, 90, 120, 150, 180 minutes) (change from baseline))
研究者
Richard Bruno
Professor and Principal Investigator
Ohio State University