Metabolic Availability of Lysine From Rice

Phase 1

Completed

• Conditions: Healthy Adults
• Interventions: Dietary Supplement: Amino Acid Crystalline Lysine Amino Acid Mix; Dietary Supplement: Cooked Rice containing Lysine

• Registration Number: NCT01474226
• Lead Sponsor: The Hospital for Sick Children

Brief Summary

The purpose of this study is to investigate the digestibility of the amino acid lysine in cooked rice in adult men using stable isotope technique.

Detailed Description

The investigators objective is to determine the metabolic availability (MA) of lysine in cooked white rice protein using the indicator amino acid oxidation (IAAO) technique. Five men will receive graded levels (20, 40, and 60, and 70%) of the lysine requirement of 35 mg/kg/d as a crystalline AA mixture,and cooked rice protein (20, 40 and 60%), respectively.

Study Timeline

• Study Start: 2011-01
• Primary Completion: 2011-11
• Study Completion: 2011-11
• Study First Submitted: 2011-11-10
• Study First Submitted QC: 2011-11-17
• Study First Posted: 2011-11-18
• Status Verified: 2013-07
• Last Update Submitted: 2013-07-26
• Last Update Posted: 2013-07-29

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 5

Inclusion Criteria

• Healthy Adult males (18 to 50years old)

Exclusion Criteria

• A history of recent weight loss or illness
• Use of any medication at the time of entry into the study.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Lysine Amino Acid	Amino Acid Crystalline Lysine Amino Acid Mix	-
Lysine Amino Acid	Cooked Rice containing Lysine	-

Primary Outcome Measures

Name	Time	Method
Metabolic Availability of lysine	45 min after intake of the fourth hourly meal (before tracer infusion))	3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment.

Secondary Outcome Measures

Name	Time	Method
Metabolic Availability of Lysine	Post Tracer Infusion (between 150 and 240 min of tracer infusion)	4 breath samples will be taken to provide F13CO2 measurement (Level of phenylalanine oxidation).Breath samples will be collected and analyzed for 13CO2 enrichment.

Trial Locations

Locations (1)

The Hospital for Sick Children; 🇨🇦 Toronto, Ontario, Canada