ROLE OF E COLI IN Colorectal Tumourigenesis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 500
- 试验地点
- 1
- 主要终点
- virulence genes: eaeA, bfpA and stx. PCR
研究概览
简要总结
Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.
详细描述
Fresh biopsy specimens have been obtained from the colonic mucosa overlying the colorectal cancer as well as from the colon of the healthy controls. Culture, genotyping and virulence of EPEC were done using (nutrient broth culture, and PCR). Strains biochemically identified as E. coli were selected from the surface of a MacConkey's plate and were serogrouped by slide agglutination tests
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •patients with colorectal carcinoma after have been diagnosed
排除标准
- •Patients with inflammatory bowel disease (IBD) or macroscopic signs of inflammation were excluded.
结局指标
主要结局
virulence genes: eaeA, bfpA and stx. PCR
时间窗: 1 week
E. coli colonies isolated from the mucosa of colorectal cancers and from healthy controls were harvested and subjected to DNA extraction (QIAGEN)
次要结局
- EPEC serogrouping:(1 week)
- Strains: OF E COLI(1 week)
研究者
MOHAMED ABDELLATIF
consultant
Mansoura University