Enteropathogenic Escherichia Coli (EPEC): Does it Have a Role in Colorectal Tumourigenesis?

Completed

• Conditions: Enteropathogenic Escherichia Coli .; Colorectal Carcinoma
• Interventions: Other: colonoscopic biopsies

• Registration Number: NCT02373020
• Lead Sponsor: Mansoura University

Brief Summary

Despite the characterization of many aetiologic genetic changes. The specific causative factors in the development of sporadic colorectal cancer remain unclear. This study was performed to detect the possible role of Enteropathogenic Escherichia coli (EPEC) in developing colorectal carcinoma.

Detailed Description

Fresh biopsy specimens have been obtained from the colonic mucosa overlying the colorectal cancer as well as from the colon of the healthy controls. Culture, genotyping and virulence of EPEC were done using (nutrient broth culture, and PCR). Strains biochemically identified as E. coli were selected from the surface of a MacConkey's plate and were serogrouped by slide agglutination tests

Study Timeline

• Study Start: 2011-01
• Primary Completion: 2013-03
• Study Completion: 2013-03
• Status Verified: 2015-02
• Study First Submitted: 2015-02-16
• Study First Submitted QC: 2015-02-25
• Study First Posted: 2015-02-26
• Last Update Submitted: 2015-04-07
• Last Update Posted: 2015-04-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 500

Inclusion Criteria

• patients with colorectal carcinoma after have been diagnosed

Exclusion Criteria

• Patients with inflammatory bowel disease (IBD) or macroscopic signs of inflammation were excluded.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
group 1	colonoscopic biopsies	colonoscopic biopsies from patients with colorectal cancer patients
(Group 2	colonoscopic biopsies	colonoscopic biopsies from healthy controls

Primary Outcome Measures

Name	Time	Method
virulence genes: eaeA, bfpA and stx. PCR	1 week	E. coli colonies isolated from the mucosa of colorectal cancers and from healthy controls were harvested and subjected to DNA extraction (QIAGEN)

Secondary Outcome Measures

Name	Time	Method
EPEC serogrouping:	1 week	Strains biochemically identified as E. coli were selected from the surface of a MacConkey's plate and were serogrouped by slide agglutination tests to detect EPEC using O-specific nonavalent E.coli antiserum (O26, O55, O86, O111, O114, O119, O124, O125, O126, O127, O128, O142), according to the manufacturer's instructions (Bio-Rad).
Strains: OF E COLI	1 week	The specimens were immediately transferred to the lab, inoculated in nutrient broth and vortexed for a few seconds then incubated overnight. The following day the broth culture was subcultured on enteric isolation media. Identification of E. coli was carried out by using standard biochemical methods.

Trial Locations

Locations (1)

Mansoura University; 🇪🇬 Mansoura, Egypt