Consistency Between Treatment Responses in PDO Models and Clinical Outcomes in Gastric Cancer

• Conditions: Gastric Cancer
• Interventions: Procedure: Tumor biopsy

• Registration Number: NCT05203549
• Lead Sponsor: D1 Medical Technology (Shanghai) Co., Ltd, China

Brief Summary

Gastric cancer is the fourth leading cause of cancer-related death worldwide. Accurate assessment of the clinical responses to current treatment regimens is key to improving the prognosis and prolonging the survival of patients. In this study, two hundred and fifty patients with gastric cancer who ought to receive neoadjuvant therapy, conversion therapy or palliative chemotherapy will be enrolled, and patient-derived organoids from their tumor biopsies will be used to test the sensitivity of chemotherapy drugs which mainly include 5-fluorouracil, irinotecan, oxaliplatin and paclitaxel.

Detailed Description

Two hundred and fifty stage II-IV gastric cancer patients who should receive neoadjuvant therapy, conversion therapy or palliative chemotherapy will be enrolled in this study. Baseline information of the enrolled patients including medical history, physical examination records and clinical examination records will be collected. Tumor material of those patients will be obtained from gastric endoscopic biopsies or surgical resection. Patient-derived organoids (PDOs) will be established and cultured from gastric cancer tumor specimens. PDOs will then be treated with drugs of the chemotherapeutic regimens for gastric cancer. Organoid size and growth will be monitored before and after the treatment, and dose-response curves will be generated. As for the assessment of clinical outcomes of patients, tumor regression grade (TRG) systems will be used to evaluate tumor histological responses, and treatment responses will also be assessed by biomedical imaging according to the Response Evaluation Criteria in Solid Tumors (RECIST1.1). Consistency between treatment responses in PDO models and clinical outcomes of patients will be assessed by correlation analysis.

Study Timeline

• Study Start: 2021-05-01
• Status Verified: 2022-01
• Study First Submitted: 2022-01-10
• Study First Submitted QC: 2022-01-10
• Last Update Submitted: 2022-01-10
• Study First Posted: 2022-01-24
• Last Update Posted: 2022-01-24
• Primary Completion: 2023-01-31
• Study Completion: 2023-06-30

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 250

Inclusion Criteria

1. Age from 18 to 70 years old, no gender limit
2. The primary tumor is diagnosed as gastric adenocarcinoma (papillary, tubular, signet ring cell, poorly differentiated and mucinous adenocarcinoma) by endoscopic biopsy sampling and follow-up histopathological staining
3. The pre-operative staging includes: a) Stage IVa（cT4bNanyM0），resectable; b) Stage II-III（cT1-2N1-3M0、cT3-4aN0-3M0）, gastro-oesophageal junction (GEJ) cancer；c) Stage III（cT3-4aN1-3M0）, non-GEJ cancer, appropriate for neoadjuvant therapy
4. Stage IVb or locally unresectable late-stage gastric or GEJ cancer, previously never received chemotherapy
5. Eastern Cooperative Oncology Group (ECOG) performance score 0 or 1
6. The function of major organs is normal, meeting the following criteria: a) routine blood tests (no blood transfusion in the past 14 days): HB≥90g/L；ANC ≥1.5×109/L；PLT ≥80×109/L；b）blood biochemical parameters: BIL <1.5-fold upper limit of normal (ULN)；ALT<2.5×ULN; AST<2.5×ULN；Crea≤1×ULN
7. Fresh tumor tissue biopsies are obtainable and the clinical information of the patients is complete
8. Patients have been informed and consented

Exclusion Criteria

1. Previously received neoadjuvant therapy
2. Previously had other types of malignancy and received chemotherapy or radiotherapy
3. Previously had chicken pox, herpes zoster or other severe contagious diseases
4. Have severe active ulcer (gastrointestinal tract, skin, etc.) or have developed a high fever
5. Have a medical history of myocardial infarction, cerebral infarction, or pulmonary embolism
6. Have complications of gastric cancer (bleeding, perforation and obstruction etc.) and need emergency operation
7. Pregnant or lactating women
8. Have severe mental diseases
9. Allergic to chemotherapy drugs
10. Have metastatic brain cancer
11. Refuse to cooperate and to complete a treatment

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Gastric cancer patients	Tumor biopsy	Patients with gastric cancer will undergo tumor biopsy before receiving neoadjuvant therapy, conversion therapy, or palliative therapy

Primary Outcome Measures

Name	Time	Method
Establishment of patient-derived organoids	2021.05 - 2023.12	Patient-derived organoids (PDOs) will be established from gastric cancer tumor specimens and the rate of successful generation of gastric cancer organoids will be calculated.

Secondary Outcome Measures

Name	Time	Method
Correlation between results of the drug sensitivity tests in patient-derived organoid models and clinical outcomes	2021.05 - 2023.12	The results from drug sensitivity tests in patient-derived organoid models will be compared with clinical outcomes after neoadjuvant therapy, conversion therapy and palliative therapy, followed by correlation analysis.

Trial Locations

Locations (1)

Zhongshan Hospital Fudan University; 🇨🇳 Shanghai, Shanghai, China