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Impact of a Probiotic-based Formula on Digestive Symptoms and Mood in Ultra Marathon Runners

Not Applicable
Completed
Conditions
Signs and Symptoms
Interventions
Dietary Supplement: Control product
Dietary Supplement: Experimental product
Registration Number
NCT06193096
Lead Sponsor
AB Biotics, SA
Brief Summary

Randomized, prospective, double-blind, placebo-controlled study in ultramarathon runners to evaluate the effects of suplementation with i3.1 probiotic formulation on the relief of common digestive symptoms associated to intense physical exercise. Secondary study outcomes aim to evaluate the effect of the probiotic formulation on fatigue, discomfort and muscle pain, as well as anxiety and mood.

Detailed Description

Gastrointestinal symptoms (GIS) are a common feature of intense exercise, especially in ultra-endurance events. The main objective of this study is to evaluate the impact of a 3-strain probiotic formulation comprising Lactiplantibacillus plantarum and Pediococcus acidilactici on digestive symptoms in a maximum of 300 runners registered in an organized ultra-marathon event. A randomized, prospective, double-blind, placebo-controlled study will be conducted. Once recruited, participants will be allocated to recieve either the probiotic or placebo (at a ratio 2:1) for 4 weeks prior to race day. During follow-up, study participants will fulfill several questionnaires at specific time points (baseline, week -3, -2, -1, -24 h previous to the race, as well as 24 h and 7 days after the race). This will be documented through a specifically designed, web-based platform. Digestive symptoms will be evaluated through validated questionnaires: modified from Pfeiffer et al (2012; main outcome) and the Gastrointestinal Symptoms Rating Scale (GSRS). Secondary objectives will comprise anxiety, mood, other symptoms (muscle pain, fatigue, respiratory symptoms), and performance in the ultra-marathon.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
277
Inclusion Criteria
  • Age >18 years
  • Correctly registered in the Salomon Ultra Pirineu 100K® 2023 race
  • Stable medication planned during the study period
  • Willing to provide informed consent and follow study procedures
  • Have signed the consent provided by the organization of the Salomon Ultra Pirineu 100K® 2023 race
Exclusion Criteria
  • Respiratory, gastrointestinal or systemic infection that has caused fever or fatigue in the 15 days prior to the study entry
  • Having taken probiotic-based supplements or dairy products with probiotics (including "Actimel" or yogurts with "bifidus") daily in the 15 days prior to the study start
  • Taking oral or parenteral antibiotics in the 15 days prior to the study start
  • History of gastrointestinal surgery within 6 months prior to the study start
  • History of cardiovascular event: angina, heart failure, myocardial infarction
  • Diabetes mellitus type 1 or 2
  • Pregnancy or lactation, or women planning to conceive during the study period
  • Chronic gastrointestinal disease: inflammatory bowel disease (Crohn's disease, ulcerative colitis), pancreatitis, short bowel syndrome
  • Gastrointestinal disease or disorders: inflammatory bowel disease (ulcerative colitis, Crohn's disease), irritable bowel syndrome, chronic diarrhea or constipation
  • Diagnosis of severe kidney disease (chronic kidney failure) or liver disease (hepatitis, cholestasis, liver failure)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Control groupControl productPlacebo formula, 1 stick/day
Experimental groupExperimental productProbiotic formula, 1 stick/day
Primary Outcome Measures
NameTimeMethod
Digestive symptoms associated to physical exerciseBaseline, +24 hours after race

Digestive tract digestive symptoms score measured with modified Pfeiffer questionnaire. Scoring ranges from 0 to 10 according to the intensity of the symptom (0-2 non-existent or mild symptoms; 3-7 moderate symptoms; 8-10 severe symptoms)

Secondary Outcome Measures
NameTimeMethod
Race time+24 hours after the race

Race time at the end of the ultra in participants who finalize the race

Competitive Anxiety before race-24 hours (before race)

Score of the Competitive State Anxiety Inventory-2R (CSAI-2R) questionnaire. The scoring ranges from 0 (nothing) to 4 (a lot).

Digestive symptoms - subscore general digestive discomfortBaseline, +24 hours after race

Evaluation of general digestive discomfort measured with modified Pfeiffer questionnaire. Scoring ranges from 0 to 10 according to the intensity of the symptom (0-2 non-existent or mild symptoms; 3-7 moderate symptoms; 8-10 severe symptoms)

Digestive symptoms - subscore other gastrointestinal symptomsBaseline, +24 hours after race

Evaluation of other gastrointestinal symptoms (nausea, dizziness, stitch) subscore measured with modified Pfeiffer questionnaire. Scoring ranges from 0 to 10 according to the intensity of the symptom (0-2 non-existent or mild symptoms; 3-7 moderate symptoms; 8-10 severe symptoms)

Digestive symptoms - subscore upper gastrointestinal symptomsBaseline, +24 hours after race

Evaluation of upper gastrointestinal symptoms subscore measured with modified Pfeiffer questionnaire. Scoring ranges from 0 to 10 according to the intensity of the symptom (0-2 non-existent or mild symptoms; 3-7 moderate symptoms; 8-10 severe symptoms)

Digestive symptoms - subscore lower gastrointestinal symptomsBaseline, +24 hours after race

Evaluation of lower gastrointestinal symptoms subscore measured with modified Pfeiffer questionnaire. Scoring ranges from 0 to 10 according to the intensity of the symptom (0-2 non-existent or mild symptoms; 3-7 moderate symptoms; 8-10 severe symptoms)

Digestive symptoms - percentage of severityBaseline, +24 hours after race

Percentage of participants with severe symptoms (value \>4) measured with modified Pfeiffer questionnaire. Scoring ranges from 0 to 10 according to the intensity of the symptom (0-2 non-existent or mild symptoms; 3-7 moderate symptoms; 8-10 severe symptoms)

Digestive symptoms measured with GSRSweek -3, -2 and -1 previous race,-24 hours before race, +7 days after race

Evaluation of digestive symptoms measured with Gastrointestinal Symptom Rating Scale (GSRS) questionnaire. The GSRS has a 7-point graded Likert-type scale where 1 represents absence of troublesome symptoms and 7 represents very troublesome symptoms

Abandonment during the race+24 hours after the race

Percentage of abandonment during the race for any reason (including gastrointestinal problems)

Adverse eventsBaseline, week -3, -2, -1, -24 hours before race, +24 hours, +7 days after race

Registration of adverse events throughout all study period

Stool consistency-24 hours before race, +24 hours after race

Evaluation of stool consistency measured through Bristol scale (score from 1 to 7). Types 1 and 2 indicate constipation, with 3 and 4 being the ideal stools as they are easy to defecate while not containing excess liquid, 5 indicating lack of dietary fiber, and 6 and 7 indicate diarrhoea

Mood States+24 hours, +7 days (after race)

Score of the total Profile of Mood States (POMS) and the associated factors: Tension, Depression, Anger, Vigor, and Fatigue, evaluated using a visual analogue scale (VAS). The scoring ranges from 0 (not at all) to 4 (extremely).

Fatigue-24 hours before race, +24 hours after race, +7 days

Fatigue, evaluated through Visual Analogue Scale (VAS) scale. The scoring ranges from 0 (not at all) to 10 (extremely).

Headache-24 hours before race, +24 hours after race, +7 days

Headache, evaluated through Visual Analogue Scale (VAS) scale. The scoring ranges from 0 (not at all) to 10 (extremely).

FeverWeek -3, -2, -1, -24 hours before race, +24 hours, +7 days after race

Number of fever episodes in a specified period of time

Muscle pain-24 hours before race, +24 hours after race, +7 days

Muscle pain, evaluated through Visual Analogue Scale (VAS) scale. The scoring ranges from 0 (not at all) to 10 (extremely).

Respiratory symptomsWeek -3, -2, -1, -24 hours before race, +24 hours, +7 days after race

Number of days with respiratory symptoms

Sleep qualityBaseline, -24 hours before race, +7 days after race

Sleep quality, evaluated through 10-point scale. The scoring ranges from 0 (the worst quality) to 10 (best quality).

Trial Locations

Locations (1)

Hospital de Cerdanya

🇪🇸

Puigcerdà, Catalonia, Spain

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