Erythrocytes and Skeletal-muscle Fatty Acids Are Positively Correlated After Caloric Restriction and Exercise

Not Applicable

Completed

• Conditions: Dietary Modification
• Interventions: Other: Caloric restriction

• Registration Number: NCT02533479
• Lead Sponsor: Centre d'Alt Rendiment

Brief Summary

The aim is compare and correlate skeletal muscle and erythrocytes fatty acids (FAs) profiles in adult healthy human after caloric restriction and acute exercise in order to validate erythrocyte FAs profile as marker of changes in muscle fatty acid composition.

Detailed Description

The study was performed with 11 healthy sportsmen participating in a caloric restriction trial for 1 month. The participants follow a caloric restriction, 30-40% respect to the usual diet of each participant, three alternate days weekly along 4 weeks. After the caloric restriction period the participants realized an exercise stress test. For each participant one blood sample and muscular biopsy were obtained after acute exercise and caloric restriction intervention. All the participants were informed of the purpose and demands of the study before giving their written consent to participate. The study protocol was in accordance with the Declaration of Helsinki for research on human participants and was approved by the local Ethics Committee of the Consell Català de l'Esport.

Study Timeline

• Study Start: 2010-01
• Primary Completion: 2012-01
• Study Completion: 2014-10
• Study First Submitted: 2015-07-07
• Status Verified: 2015-08
• Study First Submitted QC: 2015-08-25
• Last Update Submitted: 2015-08-25
• Study First Posted: 2015-08-26
• Last Update Posted: 2015-08-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 11

Inclusion Criteria

• age (18-50 years old),
• sex (male),
• equilibrate diet and physical activity of 3 days weekly.

Exclusion Criteria

• sex (female),
• smokers.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Caloric restriction	Caloric restriction	three alternate days weekly along 4 weeks.

Primary Outcome Measures

Name	Time	Method
Changes in erythrocytes FAs profiles in adult healthy human after caloric restriction and acute exercise	8 weeks	Venous blood samples were obtained from the antecubital vein of participants with vacutainers containing EDTA (ethylenediaminetetraacetic acid) as anticoagulant (6 mL) to purify erythrocytes following an adaptation of the method described elsewhere (Boyum, 1964; Sureda et al., 2006). Erythrocyte fraction was obtained after centrifugation (900 x g, 30 min, 4ºC). Then, erythrocytes were cleaned with phosphate buffered saline (PBS), centrifuged (900 x g, 20 min, 4ºC) and lysed with water at initial blood volume. Cell lysates were stored at -80ºC until FA analyses. FA composition of erythrocytes were determined by gas chromatography.
Changes in skeletal muscle FAs profiles in adult healthy human after caloric restriction and acute exercise	8 weeks	Muscular biopsy was obtained from vastus lateralis of the quadricipitalis muscle. The dermis was cut for to display muscular aponeurosis (\<2.5 cm) after clean and sterilize the biopsy zone. Next the aponeurosis was cut and a puncture with Vacora® device (Bard Biopsy Systems, Tempe, AZ, USA) was realized. The injury was cleaned and sutured, and the muscular sample was stored at -80ºC until FA analyses..FA composition of skeletal muscle were determined by gas chromatography.

Secondary Outcome Measures

Name	Time	Method
Exercise stress test energy efficiency	8 weeks	Standardized maximal treadmill exercise test
Densitometry	8 weeks	Densitometry was performed by Lunar IDXA (General Electric, USA)

Trial Locations

Locations (1)

Centre D'Alt Rendinemt, Sant Cugat; 🇪🇸 Barcelona, Spain