CD80, CD163 and CD206 Tissue Levels in Periodontal and Peri-implant Health and Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 36
- 试验地点
- 1
- 主要终点
- CD206
研究概览
简要总结
Macrophage surface markers (CD80, CD163 and CD206) will be evaluated in periodontally healthy gingiva, healthy peri-implant mucosa, and periodontitis and peri-implantitis lesions.
详细描述
Soft tissue biopsies obtained from clinically healthy gingiva, healthy peri-implant mucosa, Stage III Grade C periodontitis lesions and peri-implantitis lesions will be evaluated.
Healthy peri-implant samples are obtained from submerged implants during exposure surgery. Healthy gingiva samples are obtained from individuals receiving crown lengthening, gingivectomy or tooth extraction as excess tissue during the corresponding treatment. Samples from peri-implantitis and periodontitis (pocket depth ≥6 mm, bleeding on probing+) are obtained during initial periodontal treatment / debridement procedure with a single stroke at the pocket wall.
The samples will be grinded and ultrasonicated. Western blotting will be used for determination of CD80, CD163 and CD206 in biopsy specimens. ImageJ will be used for intensity analysis of the bands.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •diagnosed with generalized Grade C Stage III periodontitis
- •diagnosed with peri-implantitis
- •periodontally healthy individuals with dental implants in the primary healing period
- •periodontally healthy individuals who are in need of crown lengthening / gingivectomy or extraction
排除标准
- •systemic disease
- •smoking / former smokers
- •regular medicine intake
- •antibiotic or anti-inflammatory medicine intake in the last 3 months
- •pregnancy or lactation
结局指标
主要结局
CD206
时间窗: Baseline
Tissue levels of CD206 Western Blot assay analyzed using Image J software (arbitrary density units): Following scanning for optical densitometry, the gels were analyzed with Image J software by obtaining profile plots, drawing lines enclosing the interest area and measuring the peak area of interest with the wand tool. The calculated optical density values are presented as arbitrary density units.
CD80/CD206 Ratio
时间窗: Baseline
Tissue levels (Western Blot assay analyzed using Image J software) of CD80 divided by those of CD206
CD80
时间窗: Baseline
Tissue levels of CD80 Western Blot assay analyzed using Image J software: Following scanning for optical densitometry, the gels were analyzed with Image J software by obtaining profile plots, drawing lines enclosing the interest area and measuring the peak area of interest with the wand tool. The calculated optical density values are presented as arbitrary density units.
CD80/CD163 Ratio
时间窗: Baseline
Tissue levels (Western Blot assay analyzed using Image J software) of CD80 divided by those of CD163
CD163
时间窗: Baseline
Tissue levels of CD163 Western Blot assay analyzed using Image J software: Following scanning for optical densitometry, the gels were analyzed with Image J software by obtaining profile plots, drawing lines enclosing the interest area and measuring the peak area of interest with the wand tool. The calculated optical density values are presented as arbitrary density units.
次要结局
- Probing Depth(Baseline)
研究者
Mustafa YILMAZ
Asst Prof
Biruni University