Polyphenols and Overfeeding
- Conditions
- Overfeeding
- Interventions
- Dietary Supplement: 2g of grape polyphenol extract
- Registration Number
- NCT02145780
- Lead Sponsor
- Hospices Civils de Lyon
- Brief Summary
Obesity is a complex and multifactorial disease representing a major public health challenge. Indeed, overweight and obese people carry a major risk of developing insulin resistance and type 2 diabetes mellitus. It has been demonstrated that polyphenols could be used to counteract some of the mechanisms involved in the generation of insulin resistance. The model of overfeeding represents a very good study model of the metabolic complications of obesity, since it has been shown to induce a transient state of insulin resistance in the human.
The purpose of this research is to study the effects of a overfeeding (+50% of daily caloric needs over 31 days) with or without polyphenols supplementation (2g/days over 31 days) on insulin sensitivity measured in the setting of a hyperinsulinemic euglycemic clamp and on post prandial partitioning of exogenous lipids after a test meal.
During 31 days, the volunteers will consume chocolate bars, chips, chocolate breads and cola in addition to their usual diet and will be supplemented with 2g of grape polyphenols extract or with placebo.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 42
- Male
- Aged from 18 to 55 years
- Body Mass Index of 23 to 27 kg/m²
- Normal blood pressure
- Fasting glycemia < 7 mmol/L
- CRPus < 10mg/L
- Triglycerides ≤ 3mmol/L
- Medical or surgical history which may affect the results (renal -cardiovascular - hepatic- endocrine-inflammatory diseases)
- Subjects under treatment which may interfere with the measured parameters
- Eating disorder
- Intensive sportive activity
- Dairy products allergy or intolerance
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description 2g of grape polyphenol extract supplement 2g of grape polyphenol extract Men will have to consume daily 2g of grape polyphenol extract during the 31 days of overfeeding.
- Primary Outcome Measures
Name Time Method Peripheral insulin sensitivity During 31 days Volunteers will have peripheral and hepatic insulin sensitivity determined by using a two-stage hyperinsulinemic euglycemic clamp with stable isotopically labeled tracer infusion.
- Secondary Outcome Measures
Name Time Method Change in genes expression in adipose and muscle tissues Before and after the 31 days of overfeeding RT-PCR
Modification in intestinal microbiota Before and after the 31 days of overfeeding Feces analyses
Anthropometry, Fat quantification and Abdominal fat distribution During 31 days Weight, height, waist and hip circumferences, fat mass using dual x-ray absorptiometry (DEXA) and abdominal fat distribution using resonance magnetic imaging (RMI).
Fasting and Postprandial partitioning of exogenous lipid Fasting and during all the postprandial period (0-300 minutes) 13C enrichment will be measured in blood samples and breath test during a test meal performed before and after the 31 days of overfeeding.
Hepatic insulin sensitivity before and after the 31 days of overfeeding Endogenous glucose production (EGP) will be determined during the last 30 minutes of the low-dose insulin infusion.
Energy expenditure and substrate oxidation with indirect calorimetry Fasting and during all the postprandial period (0-300 minutes) Energy metabolism will be measured before and after the 31 days of overfeeding.
Trial Locations
- Locations (1)
Rhône Alpes Human Nutrition Center (CRNH Rhône-Alpes)
🇫🇷Pierre-bénite, France