To investigate the efficacy of mesenchymal stem cell conditioned media (MSCs-CM) on ameliorate maturation and developmental potential of immature human oocytes after artificial activation in the infertile female.
Not Applicable
- Conditions
- Female infertility.Female infertility
- Registration Number
- IRCT2017101736840N1
- Lead Sponsor
- Vice Chancellor for Research, Kerman University of Medical Sciences
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Complete
- Sex
- Female
- Target Recruitment
- 100
Inclusion Criteria
the age lower than 35 years old.
Exclusion criteria: endometriosis; poly cystic ovarian syndrome; abnormal chromosome; low ovarian response; chronic anovulation and fewer than five oocytes on retrieval.
Exclusion Criteria
Not provided
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Expression of the BCL2, BAX, SOD and HSP70 genes. Timepoint: after oocytes maturation. Method of measurement: by Quantitative RT-PCR.
- Secondary Outcome Measures
Name Time Method Maturity of oocytes. Timepoint: 24 and 48 hours after insertion in the medium. Method of measurement: by stereo microscope.;Developmental competence. Timepoint: 24 and 48 hours after insertion in the medium. Method of measurement: Ionomycin.