Pilot Study on DNA Repair Activity in the Skin of Day and Night Shift Workers
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 48
- 试验地点
- 1
- 主要终点
- Expression level of the DNA repair factor XPA at two times of the day in the skin of day and night shift workers.
研究概览
简要总结
The purpose of this study is to test how the skin of night shift workers responds to artificial sunlight (ultraviolet B radiation; UVB) at two different times of the day in comparison to normal day shift workers. After the skin biopsies are obtained, they will be brought to the laboratory to be exposed to UVB radiation and to measure UVB responses.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Male/Female
- •Fair skin (Fitzpatrick types I and II)
- •Age 18 to 40
- •Able to comprehend procedures/risks
- •Primarily work and are awake during normal daylight hours (6 am to 6 pm), or primarily work and are awake during night shifts hours (between the hours of 6 pm and 6 am) or may work a mixture of these shifts over the past 3 months. Rotating shifts will be categorized based on the majority of hours worked on average during the last 3 months. Able to fill out a 3-month calendar of work schedule.
排除标准
- •Known photosensitivity
- •Currently on photosensitizing medications
- •Diabetes Mellitus
- •On any hormonal agents (e.g., birth control pills)
- •History of abnormal scarring
- •History of skin infections
- •History of skin cancers
- •History of sleep disorders such as sleep apnea or insomnia
- •Known allergy to lidocaine local anesthetic
- •Pregnancy or nursing
- •Other serious health issues
研究组 & 干预措施
Day Shift Work Schedule
Day shift worker skin biopsies will be exposed to artificial sunlight (ultraviolet B radiation; UVB) at the laboratory.
干预措施: Ultraviolet B Radiation (Radiation)
Day Shift Work Schedule
Day shift worker skin biopsies will be exposed to artificial sunlight (ultraviolet B radiation; UVB) at the laboratory.
干预措施: No Ultraviolet B Radiation (Other)
Night Shift Work Schedule
Night shift worker skin biopsies will be exposed to artificial sunlight (ultraviolet B radiation; UVB) at the laboratory.
干预措施: Ultraviolet B Radiation (Radiation)
Night Shift Work Schedule
Night shift worker skin biopsies will be exposed to artificial sunlight (ultraviolet B radiation; UVB) at the laboratory.
干预措施: No Ultraviolet B Radiation (Other)
结局指标
主要结局
Expression level of the DNA repair factor XPA at two times of the day in the skin of day and night shift workers.
时间窗: After 1 hour incubation from ultraviolet B radiation exposure.
Reverse transcription quantitative PCR (RT-qPCR) and immunohistochemistry will be used to measure XPA expression at the mRNA and protein level, respectively, in skin samples obtained at 8 am and 4 pm.
Activation of DNA damage kinase signaling pathways at two times of the day in the skin of day and night shift workers.
时间窗: After 1 hour incubation from ultraviolet B radiation exposure.
Immunohistochemistry is used to measure DNA damage kinase signaling (phosphorylated Chk1 and p53) in the skin samples obtained at 8 AM and 4 PM.
Expression levels of core clock genes at two times of the day in the skin of day and night shift works.
时间窗: After 1 hour incubation from ultraviolet B radiation exposure.
Reverse Transcription Quantitative PCR (RT-qPCR) is used to measure the core circadian clock gene expression at the mRNA level in the skin samples obtained at 8 AM and 4 PM.
Activity of the nucleotide excision repair (NER) system at two times of the day in day and night shift workers.
时间窗: After 1 hour incubation from ultraviolet B radiation exposure.
A biochemical assay of nucleotide excision repair is used to measure NER in the skin samples obtained at 8 AM and 4 PM.
次要结局
未报告次要终点