A Phase 2a, Multicenter, Randomized, Adaptive, Open-label, Dose Ranging Study to Evaluate the Antiviral Effect, Safety, Tolerability and Pharmacokinetics of Cobicistat-boosted GSK2838232 Monotherapy Over 10 Days in HIV-1 Infected Treatment-naive Adults
试验速览
- 阶段
- 2 期
- 状态
- 已完成
- 入组人数
- 33
- 试验地点
- 1
- 主要终点
- Number of Participants With Vital Signs Data Outside Clinical Concern Range
研究概览
简要总结
GSK2838232 is a novel HIV-1 maturation inhibitor (MI) that is being developed for the treatment of HIV-1 infection in combination with other antiretroviral therapy (ART). This study will be a 10-day monotherapy, open-label, adaptive, dose ranging, repeat-dose study. This study will be conducted in two Parts (Part A and Part B) consisting single daily doses of GSK2838232 and Cobicistat from Day 1 to Day 10. This proof of concept open-label study will be aimed to characterize the acute antiviral activity, pharmacokinetics (PK), the relationship between PK and antiviral activity, and safety of GSK2838232/cobi administered across a range of doses over 10 days in HIV-1 infected patients. A cohort of 10 subjects will be studied in Part I followed by interim (go/no-go) analysis of Part A data. On completion of an interim analysis of part A data, further cohorts of 8 subjects will then be studied in Part B in a parallel design in two or more cohorts (depending upon the data obtained in Part A). Approximately 34 HIV-1 infected treatment-naive subjects will be enrolled during the study. Subjects in both parts will have a screening visit within 30 days prior to first dose and a follow-up visit 7-14 days after the last dose. Maximum duration of study participation will be approximately 6 Weeks.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 55 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Between 18 and 55 years of age inclusive, at the time of signing the informed consent.
- •Healthy (other than HIV infection) male or female as determined by the investigator or medically qualified designee based on a medical evaluation including medical history, physical examination, laboratory tests and cardiac monitoring, defined as no other chronic medical conditions and taking no chronic medications.
- •A subject with a clinical abnormality or laboratory parameter(s) which is/are not specifically listed in the inclusion or exclusion criteria, outside the reference range for the population being studied may be included only if the investigator in consultation with the medical monitor agree and document that the finding is unlikely to introduce additional risk factors and will not interfere with the study procedures.
- •A creatinine clearance >80 mL/minute as determined by Cockcroft-Gault equation creatinine clearance CLcr (mL/minute) = (140 - age) x weight (Wt) divided by (72 x serum creatinine [Scr]) (times 0.85 if female) where age is in years, Wt is in kilogram (kg), and Scr is in units of mg/decilitre (dL).
- •Confirmed HIV positive; CD4+ cell count >=350 cells/millimetre (mm)^3 and plasma HIV-1 RNA >=5000 copies/mL at screening.
- •No current and no prior ART.
- •Body weight >=50 kg (110 pound [lbs.]) for men and >=45 kg (99 lbs) for women and body mass index (BMI) within the range 18.5-31.0 kg/meter^2 (inclusive)
- •A female subject of reproductive or non-reproductive potential is eligible to participate if she is not pregnant (as confirmed by a negative serum or urine human chorionic gonadotrophin (hCG) test at screening and prior to first dose), not lactating, and at least one of the following conditions applies: females of reproductive potential may only be enrolled if they are using two forms of complementary contraception, which must include one barrier method. They will be counselled on safer sex practices; there is no definitive drug-drug interaction (DDI) information with GSK2838232 and an interaction with oral contraceptives is possible, so other (barrier, inter-uterine device etc.) methods of contraception will be required; fertile females, who have an established, long-term lifestyle of sexual abstinence, or only same sex partners, require no other means of birth control. Pre-menopausal females with one of the following: documented tubal ligation; documented hysteroscopic tubal occlusion procedure with follow-up confirmation of bilateral tubal occlusion; hysterectomy; documented bilateral oophorectomy; postmenopausal defined as 12 months of spontaneous amenorrhea in questionable cases a blood sample with simultaneous follicle stimulating hormone (FSH) and estradiol levels consistent with menopause. Females on hormone replacement therapy (HRT) must discontinue HRT to allow confirmation of post-menopausal status prior to study enrolment.
- •Male subjects with female partners of child bearing potential must comply with the following contraception requirements from the time of first dose of study medication until one week after the last dose of study medication; vasectomy with documentation of azoospermia; male condom plus partner use of one of the contraceptive options as: Contraceptive sub dermal implant including a <1 percent rate of failure per year; intrauterine device or intrauterine system including a <1 percent rate of failure per year; oral contraceptive, either combined or progestogen alone or injectable progestogen; contraceptive vaginal ring; percutaneous contraceptive patches. These allowed methods of contraception are only effective when used consistently, correctly and in accordance with the product label. The investigator is responsible for ensuring that subjects understand how to properly use these methods of contraception.
- •Capable of giving signed informed consent.
排除标准
- •Alanine aminotransferase (ALT) and bilirubin (BIL) >1.5 x upper limit of normal (ULN), isolated BIL >1.5xULN is acceptable if BIL is fractionated and direct BIL <35 percent.
- •Current or chronic history of liver disease, or known hepatic or biliary abnormalities (with the exception of Gilbert's syndrome or asymptomatic gallstones); hepatitis B virus (HBV) and/or hepatitis C virus (HCV) positive.
- •Subjects who have any other chronic medical condition, including cardiovascular (CV), respiratory, neurologic, psychiatric, renal, gastrointestinal (GI), oncologic, rheumatologic, or dermatologic.
- •Medical history of cardiac arrhythmias or cardiac disease or a family or personal history of long QT syndrome.
- •Unable to refrain from the use of prescription or non-prescription drugs, including vitamins, herbal and dietary supplements (including St John's Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 half-lives (whichever is longer) prior to the first dose of study medication, unless in the opinion of the Investigator and GSK medical monitor the medication will not interfere with the study procedures or compromise subject safety.
- •History of regular alcohol consumption within 6 months of the study defined as: an average weekly intake of >14 drinks for males or >7 drinks for females. One drink is equivalent to 12 grams (g) of alcohol: 12 ounces (360 mL) of beer, 5 ounces (150 mL) of wine or 1.5 ounces (45 mL) of 80 proof distilled spirits.
- •Smoking is an exclusion criteria for this study. Subject having urinary cotinine levels indicative of smoking at screening.
- •Chronic marijuana or use of other elicit medications (cocaine, heroin) is an exclusion criteria.
- •History of sensitivity to any of the study medications, or components thereof or a history of drug or other allergy that, in the opinion of the investigator or medical monitor, contraindicates their participation.
- •Presence of hepatitis B surface antigen (HBsAg), positive hepatitis C antibody test result at screening or within 3 months prior to first dose of study treatment.
- •Screening or Baseline cardiac troponin I greater than the 99 percent cutoff (>0.045 nanogram [ng]/mL by the Dimension Vista cardiac troponin [CTN] I assay).
- •A positive pre-study drug/alcohol screen.
- •Prior history of receiving an HIV maturation inhibitor
- •Where participation in the study would result in donation of blood or blood products in excess of 500 mL within 56 days.
- •The subject has participated in a clinical trial and has received an investigational product within the following time period prior to the first dosing day in the current study: 30 days, 5 half-lives or twice the duration of the biological effect of the investigational product (whichever is longer).
- •Exposure to more than four new chemical entities within 12 months prior to the first dosing day.
- •Treatment with radiation therapy or cytotoxic chemotherapeutic agents within 30 days of study drug administration or anticipated need for such treatment within the study.
- •Treatment with immunomodulating agents (such as systemic corticosteroids, interleukins, interferons) or any agent with known anti-HIV activity (such as hydroxyurea or foscarnet) within 30 days of study drug administration.
- •An active Center for Disease Control and Prevention (CDC) category C disease except cutaneous Kaposi's sarcoma not requiring systemic therapy during the trial.
- •Treatment with any vaccine within 30 days prior to receiving study medication.
- •Exclusion criteria for 24-hour screening holter: any symptomatic arrhythmia (except isolated extra systoles); sustained cardiac arrhythmias (such as atrial fibrillation, flutter or supraventricular tachycardia [>=10 seconds]); non-sustained or sustained ventricular tachycardia (defined as >=3 consecutive ventricular ectopic beats); any conduction abnormality including but not specific to left or complete bundle branch block, atrioventricular (AV) block, high grade or complete heart block Wolff-Parkinson-White (WPW) syndrome etc.; sinus pauses >3 seconds.
- •Exclusion criteria for screening ECG (a single repeat is allowed for eligibility determination): heart rate <45 and >100 beats per minute (bpm) for males, and <50 and >100 bpm for females; PR Interval <120 and >220 milliseconds (msec); QRS duration <70 and >120 msec; corrected QT (QTc) interval >450 msec; Evidence of previous myocardial infarction (Does not include ST segment changes associated with repolarization); any conduction abnormality (including but not specific to left or right complete bundle branch block, AV block [2nd degree or higher], WPW syndrome); sinus pauses >3 seconds; any significant arrhythmia which, in the opinion of the principal investigator or GSK medical monitor, will interfere with the safety for the individual subject; non-sustained or sustained ventricular tachycardia (>=3 consecutive ventricular ectopic beats).
研究组 & 干预措施
Cohort 1-GSK2838232 100 mg + Cobicistat 150 mg in Part A
During Part A (Cohort 1), subjects will receive a single dose of GSK2838232 100 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: GSK2838232 (Drug)
Cohort 1-GSK2838232 100 mg + Cobicistat 150 mg in Part A
During Part A (Cohort 1), subjects will receive a single dose of GSK2838232 100 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: Cobicistat (Drug)
Cohort 2-GSK2838232 200 mg + Cobicistat 150 mg in Part B
During Part B (Cohort 2), subjects will receive a single dose of GSK2838232 200 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: GSK2838232 (Drug)
Cohort 2-GSK2838232 200 mg + Cobicistat 150 mg in Part B
During Part B (Cohort 2), subjects will receive a single dose of GSK2838232 200 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: Cobicistat (Drug)
Cohort 3-GSK2838232 50 mg + Cobicistat 150 mg in Part B
During Part B (Cohort 3), subjects will receive a single dose of GSK2838232 50 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: GSK2838232 (Drug)
Cohort 3-GSK2838232 50 mg + Cobicistat 150 mg in Part B
During Part B (Cohort 3), subjects will receive a single dose of GSK2838232 50 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: Cobicistat (Drug)
Cohort 4-GSK2838232 20 mg + Cobicistat 150 mg in Part B
During Part B (Cohort 4), subjects will receive a single dose of GSK2838232 20 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: GSK2838232 (Drug)
Cohort 4-GSK2838232 20 mg + Cobicistat 150 mg in Part B
During Part B (Cohort 4), subjects will receive a single dose of GSK2838232 20 mg and Cobicistat 150 mg once daily each morning with a light breakfast meal and 240 mL of water from Day 1 to Day 10. Subjects will be followed up to Day 22.
干预措施: Cobicistat (Drug)
结局指标
主要结局
Number of Participants With Vital Signs Data Outside Clinical Concern Range
时间窗: Day 1 (pre-dose)
Vital signs were measured in a semi-supine position after 5 minutes rest and included temperature, systolic and diastolic blood pressure and pulse rate. The clinical concern range for vital signs were: systolic blood pressure (SBP) (low: \<85 and high: \>160 millimeters of mercury \[mmHg\]) and diastolic blood pressure (DBP) (low: \<45 and high: \>100 mmHg). Number of participants with vital signs data outside clinical concern range is presented.
Maximum Observed Concentration (Cmax) for GSK2838232 on Day 1
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 1
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Number of Participants With Clinical Chemistry Abnormalities of Potential Clinical Importance
时间窗: Up to Day 22
Blood samples were collected for the assessment of clinical chemistry parameters. The clinical concern range for the parameters were: carbon dioxide/bicarbonate (low: \<18 millimoles per liter \[mmol/L\] and high: \>32 mmol/L); urea (high: \>9 mmol/L); creatinine (high: change from Baseline \>44.2 micromoles per liter \[µmol/L\]), glucose (low: \<3 and high: \>9 mmol/L); potassium (low: \<3 and high: \>5.5 mmol/L); troponin I (high: \>=0.01 micrograms per liter \[µg/L\]) and sodium (low: \<130 mmol/L and high: \>150 mmol/L). Data for any visit post-Baseline is reported.
Number of Participants With Abnormal Urine Parameters
时间窗: Up to Day 22
Urine samples were collected for the assessment of following urine parameters by dipstick method: pH, glucose, protein, blood and ketones. The number of participants with abnormal urine parameters is presented.
Number of Participants With Abnormal Electrocardiogram (ECG) Findings
时间窗: Up to Day 22
Triplicate 12-lead ECGs were obtained using an ECG machine that automatically calculated the heart rate and measured PR, QRS, QT, and corrected QT (QTc) intervals. Number of participants with abnormal ECG findings at worst-case post Baseline is presented.
Area Under the Plasma Concentration Time Curve From Zero (Pre-dose) to 24 Hours (AUC[0 to 24]) for GSK2838232 on Day 1
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 1
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232. The analysis was performed on Pharmacokinetic Population which comprised of participants who received GSK2838232 and underwent plasma pharmacokinetic sampling during the study.
Pre-dose Concentration (C0) of GSK2838232 on Day 10
时间窗: Pre dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Cmax for GSK2838232 on Day 10
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Terminal Elimination Half-life (T1/2) of GSK2838232 Following Administration on Day 10
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Number of Participants With Liver Function Laboratory Abnormalities of Potential Clinical Importance
时间窗: Up to Day 22
Blood samples were collected for the assessment of liver function parameters. The clinical concern range for liver function parameters were: albumin (low: \<30 g/L), total protein (low: \<15 and high: \>15 g/L), alanine aminotransferase (high: \>=2 times upper limit of normal \[ULN\]); aspartate aminotransferase (high: \>=2 times ULN); alkaline phosphatase (high: \>=2 times ULN); total bilirubin (high: \>=1.5 times ULN); direct bilirubin (high: \>0.3 times ULN).
Area Under the Concentration-time Curve Over the Dosing Interval (AUC [0 to Tau]) for GSK2838232 on Day 10
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Maximum Decline From Baseline in Plasma HIV-1 Ribonucleic Acid (RNA)
时间窗: Baseline (Day 1) to Day 21
Plasma samples were collected for quantitative analysis of plasma HIV-1 RNA. An HIV-1 RNA polymerase chain reaction (PCR) assay with a lower limit of detection (LLOD) of 50 copies/milliliter (ultrasensitive assay) was used for post-baseline assessments. Baseline value was the value at latest pre-dose assessment. The maximum decline was determined using change from Baseline in plasma HIV-RNA values at each time point. The analysis was performed on Intent To Treat (ITT) Population which comprised of all participants who met study criteria and were enrolled into the study with documented evidence of having received at least 1 dose of treatment and at least one post-Baseline HIV-1 RNA measurement.
Number of Participants Who Were Administered Concomitant Medications
时间窗: Up to Day 22
Concomitant medications (prescription and non-prescription) were administered only as medically necessary during the study. Number of participants who received any concomitant medications is presented.
Time to Maximum Observed Concentration (Tmax) for GSK2838232 on Day 1
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 1
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Absorption Lag Time (Tlag) for GSK2838232 on Day 1
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 1
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Concentration at End of Dosing Interval (Ctau) for GSK2838232 on Day 10
时间窗: 24 hours post-dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Tmax for GSK2838232 Following Administration on Day 10
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Number of Participants With Adverse Events (AEs) and Serious Adverse Events (SAEs)
时间窗: Up to Day 22
An AE is any untoward medical occurrence in a participant or clinical investigation participant, temporally associated with the use of a medicinal product, whether or not considered related to the medicinal product. SAE is defined as any untoward medical occurrence that, at any dose: results in death; is life threatening; requires hospitalization or prolongation of existing hospitalization; results in disability/incapacity; is a congenital anomaly/birth defect; other important medical events; is associated with liver injury and impaired liver function. Safety Population comprised of all participants who received at least one dose of study treatment.
Number of Participants With Hematology Parameter Abnormalities of Potential Clinical Importance
时间窗: Up to Day 22
Blood samples were collected for the assessment of hematology parameters. The clinical concern range for the parameters were: hematocrit (high: \>0.54 proportion of red blood cells in blood); hemoglobin (high: \>180 grams per liter \[g/L\]), lymphocytes (low: \<0.8x10\^9 cells/L); neutrophil count (low: \<1.5x10\^9 cells/L); platelet count (low: \<100x10\^9 cells/L and high: \>550x10\^9 cells/L); white blood cells (low: \<3x10\^9 cells/L and high: \>20x10\^9cells/L). Data for any visit post-Baseline is reported.
Concentration of GSK2838232 at 24 Hours Post-dose on Day 1
时间窗: 24 hours post-dose on Day 1
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
Apparent Oral Clearance of GSK2838232 Following Administration on Day 10
时间窗: Pre dose and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Day 10
Serial plasma samples were collected at indicated time points for pharmacokinetic analysis of GSK2838232.
次要结局
- Dose Proportionality of GSK2838232(pre dose, and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Days 1 and 10)
- Change From Baseline to Day 11 in log10 Plasma HIV-1 RNA Relative to Day 10 AUC (0 to Tau)(Baseline (Day 1), Days 10 and 11)
- Change From Baseline in Cluster of Differentiation 4+ (CD4+) Cell Count to Day 11(Baseline (Day 1) and Day 11)
- Change From Baseline to Day 11 in CD4+ Count Relative to Day 10 AUC (0 to Tau)(Baseline (Day 1), Days 10 and 11)
- Change From Baseline to Day 11 in log10 Plasma HIV-1 RNA Relative to Day 10 Ctau(Baseline (Day 1), Days 10 and 11)
- Change From Baseline to Day 11 in CD4+ Count Relative to Day 10 Ctau(Baseline (Day 1), Days 10 and 11)
- Change From Baseline to Day 11 in log10 Plasma HIV-1 RNA Relative to Day 10 Cmax(Baseline (Day 1), Days 10 and 11)
- Change From Baseline to Day 11 in CD4+ Count Relative to Day 10 Cmax(Baseline (Day 1), Days 10 and 11)
- Pre-morning Dose Concentrations (C0) on Day 2 Through 11(Pre-dose on Days 2, 3, 4, 5, 8, 9, 10 and 11)
- Number of Participants With Emergent Drug Resistance Mutations(Up to Day 11)
- Accumulation Ratio for GSK2838232(pre dose, and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12 and 24 hours post-dose on Days 1 and 10; pre-dose on Days 3, 4, 5, 8 and 9; Days 12 and 14)
- Steady State Assessment of Plasma Pre-dose Concentrations by Treatment(Pre-dose on Days 8, 9 and 10)