Vitamin D Receptor, Interleukin-1A and Interleukin-1B Gene Polymorphisms in Periodontitis

Completed

• Conditions: Periodontitis; Polymorphism, Restriction Fragment Length

• Registration Number: NCT04806971
• Lead Sponsor: Marmara University

Brief Summary

Background: The aim of the study is to find out the prevelance of VDR (rs731236), IL1B (rs1143634) and IL1A (rs1800587)gene polymorphisms in Turkish population and their association with stage III grade B / C periodontitis.

Methods: Individuals who were found to be systemically and periodontally healthy (N = 100) after clinical and radiographic examination and who were diagnosed with Stage III Grade B / C periodontitis (N = 100) were included in the study. Gingival index, Plaque index, bleeding on probing, clinical attachment level and pocket depths of the patients were recorded. subjects genotyped for rs731236, rs1143634 and rs1800587 gene polymorphisms with Real Time Polymerase Chain Reaction.

Detailed Description

In this study we recruited 200 participants from the Periodontology Clinics. Periodontal clinical parameters such as plaque index, gingival index, probing depth and clinical attachment level were assessed. Genotyping rs731236, rs1800587 and rs1143634 gene polymorphisms was performed by Real Time Polymerase Chain Reaction.Statistical analysis was done by the statistical software package SPSS v16. SNPassoc.

Study Timeline

• Study Start: 2018-01-29
• Primary Completion: 2019-09-27
• Study Completion: 2019-09-27
• Study First Submitted: 2021-03-16
• Study First Submitted QC: 2021-03-18
• Study First Posted: 2021-03-19
• Status Verified: 2021-11
• Last Update Submitted: 2021-11-30
• Last Update Posted: 2021-12-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 200

Inclusion Criteria

systemically healthy, previously untreated periodontitis patients

Exclusion Criteria

presence of systemic disease such as diabetes, rheumatic fever, liver or kidney diseases, neurologic deficiencies, immunologic diseases; pregnancy; smoking; receive of any medication which may affect periodontal tissues; used antibiotics for any purpose within 6 months.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Vitamin D Receptor gene polymorphism	baseline
Interleukin-1B gene polymorphism	baseline
IL-1A gene polymorphism	baseline

Trial Locations

Locations (2)

Marmara University; 🇹🇷 İstanbul, Maltepe, Turkey

Marmara University Dental Faculty Periodontology Department; 🇹🇷 İstanbul, Turkey